Gorgadze Oleg, Fanelli Elena, Vovlas Alessio, Troccoli Alberto, Tarasco Eustachio, De Luca Francesca
Institute of Zoology, Ilia State University, Tbilisi 0162, Georgia.
Institute for Sustainable Plant Protection-CNR, Via Amendola 122/D, 70126 Bari, Italy.
Biology (Basel). 2025 May 7;14(5):512. doi: 10.3390/biology14050512.
Surveys were conducted in the village of Shamgona in the Samegrelo Region of West Georgia, and two nematode species belonging to the genus and a population of were isolated. Integrative diagnosis, combining morphology, PCA, sequencing and phylogeny, was carried for sp. from the soil of hazelnut orchard. This population shows high similarity at morphological and morphometrical levels with from Iran, and the main distinctive features with from Iran are the presence of eight lateral lines and males. Sequence and phylogenetic analyses by using ITS, D2-D3 expansion domains of the 28S rRNA gene, the partial 18S rRNA gene, and the mitochondrial COI confirmed the identification as A second population of sp. and was characterized only at the molecular and phylogenetic level. The phylogenetic analyses, based on ITS and D2-D3 expansion domains, revealed the occurrence of in Georgia grouping with sequences with high support. The phylogenetic trees of , based on D2-D3 expansion domains, ITS, the partial 18S rRNA gene, and mitochondrial COI confirmed the high variability among geographical populations. The present study reports on the occurrence of two species of in Georgia for the first time.
在格鲁吉亚西部Samegrelo地区的沙姆戈纳村进行了调查，分离出了属于该属的两种线虫以及一个种群。对榛子果园土壤中的某物种进行了综合诊断，综合了形态学、主成分分析、测序和系统发育分析。该种群在形态和形态测量水平上与来自伊朗的某物种高度相似，与来自伊朗的该物种的主要区别特征是存在八条侧线和雄性。通过使用ITS、28S rRNA基因的D2 - D3扩展域、部分18S rRNA基因和线粒体COI进行序列和系统发育分析，证实其鉴定为某物种。第二个某物种和另一物种的种群仅在分子和系统发育水平上进行了表征。基于ITS和D2 - D3扩展域的系统发育分析表明，格鲁吉亚存在某物种，与具有高支持度的序列聚在一起。基于D2 - D3扩展域、ITS、部分18S rRNA基因和线粒体COI构建的某物种系统发育树证实了地理种群之间的高度变异性。本研究首次报道了格鲁吉亚存在两种某物种。
