Gorgadze O, Troccoli A, Fanelli E, Tarasco E, De Luca F
Institute of Zoology of Ilia State University, Tbilisi, 0162, Georgia.
Institute for Sustainable Plant Protection-CNR, Via Amendola 122/D, 70126, Bari, Italy.
J Nematol. 2020 Aug 31;52. doi: 10.21307/jofnem-2020-081. eCollection 2020.
During a field survey for entomopathogenic nematodes in Georgia, in the territory of Borjomi-Kharagauli, a nematode population was isolated from the body of single dead beetle of Motschulsky 1845 (Coleoptera: Lucanidae). Based on morphological characters and molecular analyses, the nematode species is identical to (Schneider, 1860, 1866), being the first record of this species in Georgia. Morphometrics of the Georgian population agree with the original description, and females differ from males by very few characters. The Georgian population of was molecularly characterized by sequencing the D2 to D3 expansion domains of the 28S rRNA gene and two new molecular markers as the ITS and the mitochondrial COI gene. Phylogenetic analyses revealed that all sequences grouped together along with all other species.
在格鲁吉亚博尔若米-哈拉加乌利地区进行昆虫病原线虫实地调查期间,从一只1845年的莫氏锹甲(鞘翅目:锹甲科)死虫体内分离出一个线虫种群。基于形态特征和分子分析,该线虫物种与(施奈德,1860年,1866年)相同,这是该物种在格鲁吉亚的首次记录。格鲁吉亚种群的形态测量结果与原始描述一致,雌性与雄性的差异很小。通过对28S rRNA基因的D2至D3扩展域以及两个新的分子标记ITS和线粒体COI基因进行测序,对格鲁吉亚的 种群进行了分子特征分析。系统发育分析表明,所有 序列与所有其他 物种一起聚类。