Hu Qingxi, Hou Xiaoyang, Shi Hekai, Song Yongteng, Zhou Bing, Hu Xinli, Zhang Haiguang, Gu Yan
Rapid Manufacturing Engineering Center, School of Mechatronical Engineering and Automation, Shanghai University, Shanghai 200444, China.
National Demonstration Center for Experimental Engineering Training Education, Shanghai University, Shanghai 200444, China.
Bioengineering (Basel). 2025 May 14;12(5):522. doi: 10.3390/bioengineering12050522.
Tension-free hernioplasty has effectively reduced postoperative recurrence and mitigated complications by employing polymer patches. However, clinically used polymer patches often fall short in terms of the anti-deformation, anti-adhesion, and tissue integration functions, which can result in visceral adhesions and foreign body reactions after implantation. In this study, a Janus three-layer composite patch was developed for abdominal wall defect repair using a combination of 3D printing, electrospraying, and electrospinning technologies. On the visceral side, a dense electrospun polyvinyl alcohol/sodium hyaluronate (PVA/HA) scaffold was fabricated to inhibit cell adhesion. The middle layer, composed of polycaprolactone (PCL), provided mechanical support. On the muscle-facing side, a loose and porous electrospun nanofiber scaffold was created through electrospraying and electrospinning, promoting cell adhesion and migration to facilitate tissue regeneration. Mechanical testing demonstrated that the composite patch possessed excellent tensile strength (23.58 N/cm), surpassing the clinical standard (16 N/cm). Both in vitro and in vivo evaluations confirmed the patch's outstanding biocompatibility. Compared with the control PCL patch, the Janus composite patch significantly reduced the visceral adhesion and enhanced the tissue repair in animal models. Collectively, this Janus composite patch integrated anti-deformation, anti-adhesion, and tissue-regenerative properties, providing a promising solution for effective abdominal wall defect repair.
无张力疝修补术通过使用聚合物补片有效地降低了术后复发率并减轻了并发症。然而，临床使用的聚合物补片在抗变形、抗粘连和组织整合功能方面往往存在不足，这可能导致植入后出现内脏粘连和异物反应。在本研究中，利用3D打印、电喷雾和静电纺丝技术相结合，开发了一种用于腹壁缺损修复的双面三层复合补片。在内脏侧，制备了致密的静电纺聚乙烯醇/透明质酸钠（PVA/HA）支架以抑制细胞粘附。中间层由聚己内酯（PCL）组成，提供机械支撑。在面向肌肉的一侧，通过电喷雾和静电纺丝制备了疏松多孔的静电纺纳米纤维支架，促进细胞粘附和迁移以利于组织再生。力学测试表明，该复合补片具有优异的拉伸强度（23.58 N/cm），超过了临床标准（16 N/cm）。体外和体内评估均证实了该补片具有出色的生物相容性。与对照PCL补片相比，双面复合补片在动物模型中显著减少了内脏粘连并增强了组织修复。总的来说，这种双面复合补片兼具抗变形、抗粘连和组织再生特性，为有效的腹壁缺损修复提供了一种有前景的解决方案。
