Jin Ziqiang, Chen Xiaoling, Du Zechuan, Yuan Yi, Li Xiaoli, Xie Ping
Institute of Electric Engineering, Yanshan University, Qinhuangdao 066004, China.
Key Laboratory of Intelligent Rehabilitation and Neuromodulation of Hebei Province, Yanshan University, Qinhuangdao 066004, China.
Bioengineering (Basel). 2025 May 17;12(5):541. doi: 10.3390/bioengineering12050541.
(1) Background: Ischemic stroke is a major global public-health concern with complex pathogenesis. Current treatment strategies face challenges. Low-intensity pulsed ultrasound stimulation (LIPUS), a non-invasive neuromodulation technology, shows promise in treating ischemic stroke, yet its underlying mechanisms lack in-depth investigation, especially in quantitative efficacy evaluation. (2) Methods: This study aimed to develop a neuromuscular functional coupling-based dynamic time warping (DTW) model to evaluate LIPUS's neuroprotective effects in a mouse model of ischemic stroke. A bilateral carotid artery occlusion (BCAO) model in mice was established, and LIPUS treatment was given. Time- and frequency-domain analyses of local field potentials (LFPs) and electromyography (EMG) were conducted, and outcomes were quantified using a percentage-based scoring system. (3) Results: The BCAO+LIPUS group scored significantly higher than the BCAO group. (4) Conclusions: This study demonstrated that LIPUS is neuroprotective in BCAO mice and that the DTW-100 assessment evaluation model can quantify the neuroprotective effects of LIPUS.
(1) 背景：缺血性中风是一个全球性的重大公共卫生问题，其发病机制复杂。目前的治疗策略面临挑战。低强度脉冲超声刺激（LIPUS）作为一种非侵入性神经调节技术，在治疗缺血性中风方面显示出前景，但其潜在机制缺乏深入研究，尤其是在定量疗效评估方面。(2) 方法：本研究旨在开发一种基于神经肌肉功能耦合的动态时间规整（DTW）模型，以评估LIPUS在缺血性中风小鼠模型中的神经保护作用。建立小鼠双侧颈动脉闭塞（BCAO）模型，并给予LIPUS治疗。对局部场电位（LFP）和肌电图（EMG）进行时域和频域分析，并使用基于百分比的评分系统对结果进行量化。(3) 结果：BCAO+LIPUS组的评分显著高于BCAO组。(4) 结论：本研究表明LIPUS对BCAO小鼠具有神经保护作用，且DTW-100评估模型可量化LIPUS的神经保护作用。
