Orthodontic temporary anchorage devices (TADs) in the lateral maxillary region are useful tools for successful orthodontic treatment. Radiological anatomical knowledge is crucial for the successful placement of TADs. The use of cone-beam computed tomography (CBCT) is essential for evaluating the relationship between the ideal placement point (IPP) and dental structures, particularly in cases with anatomical limitations. Accordingly, this study aims to assess the anatomical conditions for orthodontic mini-implant (MI) insertion in the posterior maxilla using CBCT as the gold standard. This retrospective study included 62 patients (37.1% male, 62.9% female) aged 11 to 50 years. CBCT scans (sagittal and axial cross-sections) were used to evaluate interdental bone characteristics in different regions. The evaluated regions were defined as follows: Region 1 (canine and first premolar), Region 2 (first and second premolars), Region 3 (second premolar and first molar), and Region 4 (first and second molars). All parameters were assessed at three predefined levels: A, B, and C, located 4, 3, and 2 mm, respectively, from the alveolar crest. At the aforementioned levels, we performed measurements, such as the interdental width (IDW) in the mesiodistal direction and buccopalatal depth (BPD). The last observation was the relationship between the ideal TAD placement point (IPP) and dental structures, such as contact points (CPs) and cusp tips (C1-cusp of mesial tooth, C2-cusp of distal tooth, in each region). A statistically significant positive correlation was found between the IDW and BPD at Levels A, B, and C in Region 1, while a negative correlation was observed between the IDW and BPD at Level C in Region 2'. The highest percentages of IDW exceeding 3 mm were found in Region 4 at Level A (67.7%), followed by Region 1' and 2', both at Level A. The mean interdental width measured at each level on the right and left sides was highest at Level A, exceeding 3 mm, and the width decreased with each successive level. The mean BPD measured at each level on the right and left sides was also highest at Level A. This methodological approach could assist in ensuring precise and efficient implant insertion. Furthermore, it can be concluded that the safe zone for buccal and interdental mini-implant placement is located 4 mm from the alveolar crest at Level A. Also, the CBCT analysis algorithm may serve as a valuable tool for clinicians in determining optimal TAD placement in different dental regions.