Phylogenomic and Evolutionary Insights into Lipoprotein Lipase (LPL) Genes in Tambaqui: Gene Duplication, Tissue-Specific Expression and Physiological Implications.

BACKGROUND/OBJECTIVES: Lipoprotein lipase (LPL) is a key enzyme in lipid metabolism, crucial for the hydrolysis of triglycerides in lipoproteins and maintaining lipid homeostasis in vertebrates. This study aims to characterize the lipoprotein lipase genes in the tambaqui () genome, investigating their evolutionary history from a phylogenomic perspective.

METHODS

Phylogenetic and syntenic analyses were used to identify the gene copies in the tambaqui genome and expression patterns were examined across different tissues. A comparative analysis with genes from other vertebrates was also conducted to assess evolutionary relationships and functional diversification.

RESULTS

We identified three gene copies in the tambaqui genome: , , and the lesser-known member of the lipoprotein lipase subfamily, . These proteins possess conserved sites essential for lipoprotein lipase function, with variations that may affect their physicochemical properties and lipolytic activity. Key amino acid variations, such as in the lid region and glycosylation sites, were observed among orthologs. Gene expression analysis showed high and expression in the liver, and expression in the gonads, suggesting tissue-specific roles. Comparative analysis revealed distinct expression patterns among teleost fish, with tambaqui exhibiting a unique profile consistent with its migratory lifestyle and varied diet.

CONCLUSIONS

This study offers new insights into the evolution and functional diversification of lipoprotein lipases in vertebrates, highlighting the complexity of lipid metabolism in fish. These findings contribute to understanding the adaptability of teleost fish to diverse environments and lay the foundation for future research in lipid metabolism regulation, including Neotropical species, with potential applications in aquaculture and conservation.