Zhang Fanxue, Mu Meimei, Wang Zonglin, Zhang Haoran, Song Yilan, Xiao Rong
Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Institute of Entomology, Guizhou University, Guiyang 550025, China.
Insects. 2025 Apr 24;16(5):446. doi: 10.3390/insects16050446.
(Meyrick) is an important invasive pest that seriously affects the yield and quality of tomatoes. In this study, based on the previously obtained transcriptome data, a total of 25,123 SSR loci and 332,537 SNP loci were identified. The identified SSRs had occurrence and appearance frequencies of 28.62% and 40.60%, respectively. SSRs with a length equal to or greater than 12 bp constituted 58.25% of the total SSR loci. Through the analysis of GO, COG annotations, and the KEGG pathway database, it was revealed that the majority of SSR-transcripts were involved in fundamental cellular metabolic functions. In addition, the frequency of SNP occurrence was approximately one SNP locus per 175 base pairs (bp), and the transition type was the main variant type. The functional annotations of SNP-transcripts were primarily concentrated in biological synthesis pathways such as peroxisome, RNA transport, carbon metabolism, and protein processing in the endoplasmic reticulum. These synthesis pathways are involved in the detoxification mechanism of and contribute to its enhanced survival under pesticide susceptibility. These findings provide valuable data for constructing genetic maps, assessing genetic diversity, and determining functional orientation in insects, and they also provide basic data for the molecular mechanism of 's response to pesticide susceptibility.
（麦蛾）是一种重要的入侵害虫，严重影响番茄的产量和品质。在本研究中，基于先前获得的转录组数据，共鉴定出25123个SSR位点和332537个SNP位点。所鉴定的SSR的出现频率和外观频率分别为28.62%和40.60%。长度等于或大于12 bp的SSR占总SSR位点的58.25%。通过对GO、COG注释和KEGG通路数据库的分析发现，大多数SSR转录本参与基本的细胞代谢功能。此外，SNP出现的频率约为每175个碱基对（bp）有一个SNP位点，且转换类型是主要的变异类型。SNP转录本的功能注释主要集中在过氧化物酶体、RNA转运、碳代谢和内质网中的蛋白质加工等生物合成途径。这些合成途径参与了（某种昆虫，原文未明确）的解毒机制，并有助于其在农药敏感性下提高存活率。这些发现为构建昆虫遗传图谱、评估遗传多样性和确定功能方向提供了有价值的数据，也为（某种昆虫，原文未明确）对农药敏感性反应的分子机制提供了基础数据。
