Liu Min, Xiao Feng, Zhu Jiayun, Fu Di, Wang Zonglin, Xiao Rong
Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Institute of Entomology, Guizhou University, Guiyang 550025, China.
Insects. 2023 Apr 6;14(4):363. doi: 10.3390/insects14040363.
(Meyrick) is a devastating invasive pest worldwide. The abamectin and chlorantraniliprole complex have become an alternative option for chemical control because they can enhance insecticidal activity and delay increased drug resistance. Notably, pests are inevitably resistant to various types of insecticides, and compound insecticides are no exception. To identify potential genes involved in the detoxification of abamectin and chlorantraniliprole complex in , PacBio SMRT-seq transcriptome sequencing and Illumina RNA-seq analysis of abamectin and chlorantraniliprole complex-treated were performed. We obtained 80,492 non-redundant transcripts, 62,762 (77.97%) transcripts that were successfully annotated, and 15,524 differentially expressed transcripts (DETs). GO annotation results showed that most of these DETs were involved in the biological processes of life-sustaining activities, such as cellular, metabolic, and single-organism processes. The KEGG pathway enrichment results showed that the pathways related to glutathione metabolism, fatty acid and amino acid synthesis, and metabolism were related to the response to abamectin and chlorantraniliprole complex in . Among these, 21 P450s were differentially expressed (11 upregulated and 10 downregulated). The qRT-PCR results for the eight upregulated P450 genes after abamectin and chlorantraniliprole complex treatment were consistent with the RNA-Seq data. Our findings provide new full-length transcriptional data and information for further studies on detoxification-related genes in .
（Meyrick）是一种在全球范围内极具破坏性的入侵害虫。阿维菌素和氯虫苯甲酰胺复配剂已成为化学防治的一种替代选择，因为它们可以增强杀虫活性并延缓耐药性的增加。值得注意的是，害虫不可避免地会对各种类型的杀虫剂产生抗性，复合杀虫剂也不例外。为了鉴定参与阿维菌素和氯虫苯甲酰胺复配剂解毒的潜在基因，对经阿维菌素和氯虫苯甲酰胺复配剂处理的[害虫名称未给出]进行了PacBio SMRT-seq转录组测序和Illumina RNA-seq分析。我们获得了80492条非冗余转录本中，62762条（77.97%）转录本成功注释，以及15524条差异表达转录本（DETs）。GO注释结果表明，这些DETs中的大多数参与了维持生命活动的生物学过程，如细胞、代谢和单生物体过程。KEGG通路富集结果表明，与谷胱甘肽代谢、脂肪酸和氨基酸合成以及代谢相关的通路与[害虫名称未给出]对阿维菌素和氯虫苯甲酰胺复配剂的反应有关。其中，21个P450基因差异表达（11个上调，10个下调）。阿维菌素和氯虫苯甲酰胺复配剂处理后8个上调P450基因的qRT-PCR结果与RNA-Seq数据一致。我们的研究结果为进一步研究[害虫名称未给出]中与解毒相关的基因提供了新的全长转录数据和信息。
