Nature-Identical Safranal and Dihydrocoumarin from ((Spreng., 1970) King and H. Rob.) Target Energy Metabolism to Control Buren, 1972 (Hymenoptera: Formicidae).

The bio-prospecting of bioactive phytochemicals from invasive flora presents a sustainable paradigm for the ecologically conscious management of major invasive pest . , while recognized for its allelopathic insecticidal properties, exhibits poorly characterized toxicological profiles and mechanistic underpinnings against , warranting systematic investigation to elucidate its mode of action. This study elucidates the bioactive insecticidal compounds of and their toxicological impacts on , including behavioral, metabolic, and enzymatic perturbations, via liquid chromatography-mass spectrometry (LC-MS) profiling. The ethanol extracts of the roots, stems, and leaves of have shown control effects on , with an LC (50% lethal concentration) of 331.847, 188.256, and 166.253 mg/mL at 48 h, respectively. Metabolite profiling of revealed that safranal and dihydrocoumarin are relatively high in plant leaves, and they showed significant insecticidal activity and behavioral inhibitory effects on with LC 349.042 mg/L and 118.336 mg/L at 48 h, respectively. Notably, these two bioactive compounds disrupted the normal energy production through glucose metabolism and the citrate cycle, which eventually led to the death of . Further, these two compounds also activated the detoxification metabolic pathway of . These findings provide a theoretical basis for the use of these bioactive compounds in the integrated management of and may lead to the development of a new biopesticide.