Wu Mingqi, Luo Rongchao, Hussain Mehboob, Wu Wenmei, Li Shini, Guo Zijun, Jia Boyu, Bi Gaofeng, Gao Xi, Wu Guoxing, Qin Deqiang
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, China.
School of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Insects. 2025 May 20;16(5):540. doi: 10.3390/insects16050540.
The bio-prospecting of bioactive phytochemicals from invasive flora presents a sustainable paradigm for the ecologically conscious management of major invasive pest . , while recognized for its allelopathic insecticidal properties, exhibits poorly characterized toxicological profiles and mechanistic underpinnings against , warranting systematic investigation to elucidate its mode of action. This study elucidates the bioactive insecticidal compounds of and their toxicological impacts on , including behavioral, metabolic, and enzymatic perturbations, via liquid chromatography-mass spectrometry (LC-MS) profiling. The ethanol extracts of the roots, stems, and leaves of have shown control effects on , with an LC (50% lethal concentration) of 331.847, 188.256, and 166.253 mg/mL at 48 h, respectively. Metabolite profiling of revealed that safranal and dihydrocoumarin are relatively high in plant leaves, and they showed significant insecticidal activity and behavioral inhibitory effects on with LC 349.042 mg/L and 118.336 mg/L at 48 h, respectively. Notably, these two bioactive compounds disrupted the normal energy production through glucose metabolism and the citrate cycle, which eventually led to the death of . Further, these two compounds also activated the detoxification metabolic pathway of . These findings provide a theoretical basis for the use of these bioactive compounds in the integrated management of and may lead to the development of a new biopesticide.
从入侵植物中生物勘探生物活性植物化学物质，为具有生态意识的主要入侵害虫管理提供了一种可持续模式。[植物名称]虽然因其化感杀虫特性而闻名，但其毒理学特征和针对[害虫名称]的作用机制尚未得到充分表征，需要进行系统研究以阐明其作用方式。本研究通过液相色谱 - 质谱（LC - MS）分析，阐明了[植物名称]的生物活性杀虫化合物及其对[害虫名称]的毒理学影响，包括行为、代谢和酶学扰动。[植物名称]根、茎和叶的乙醇提取物对[害虫名称]显示出防治效果，在48小时时的LC（50%致死浓度）分别为331.847、188.256和166.253毫克/毫升。[植物名称]的代谢物分析表明，藏红花醛和二氢香豆素在植物叶片中相对含量较高，它们对[害虫名称]分别表现出显著的杀虫活性和行为抑制作用，在48小时时的LC分别为349.042毫克/升和118.336毫克/升。值得注意的是，这两种生物活性化合物通过葡萄糖代谢和柠檬酸循环扰乱了正常的能量产生，最终导致[害虫名称]死亡。此外，这两种化合物还激活了[害虫名称]的解毒代谢途径。这些发现为这些生物活性化合物在[害虫名称]综合管理中的应用提供了理论基础，并可能导致新型生物农药的开发。
