Fu Li, Li Penghui, Rui Zhiyi, Sun Jiang, Yang Jun, Wang Yuanxin, Jia Dong, Hu Jun, Li Xianchun, Ma Ruiyan
College of Plant Protection, Shanxi Agricultural University, Jinzhong 030801, China.
Department of Entomology and BIO5 Institute, University of Arizona, Tucson, AZ 85721, USA.
Int J Mol Sci. 2025 May 10;26(10):4586. doi: 10.3390/ijms26104586.
is the most effective natural enemy for the control of the invasive weed (Mart.) Griseb. However, research on the gene function and potential genetic improvement of is limited due to a lack of effective genetic tools. In this study, we employed the () gene as a target gene to develop a CRISPR/Cas9-based gene editing method applicable to . We showed that injection of Cas9/sgRNA ribonucleoprotein complexes (RNPs) of the gene into pre-blastoderm eggs induced genetic insertion and deletion mutations, leading to white eyes. Our results demonstrate that CRISPR/Cas9-mediated gene editing is possible in , offering a valuable tool for studies of functional genomics and genetic improvement of , which could potentially lead to more effective control of invasive weeds through the development of improved strains of this biocontrol agent. In addition, the white-eyed mutant strain we developed could potentially be useful for other transgenic research studies on this species.
是控制入侵杂草（Mart.）Griseb.的最有效天敌。然而，由于缺乏有效的遗传工具，对其基因功能和潜在遗传改良的研究有限。在本研究中，我们以（）基因作为靶基因，开发了一种适用于的基于CRISPR/Cas9的基因编辑方法。我们发现，将该基因的Cas9/sgRNA核糖核蛋白复合物（RNPs）注射到胚盘前期卵中会诱导基因插入和缺失突变，导致白眼。我们的结果表明，CRISPR/Cas9介导的基因编辑在中是可行的，为功能基因组学研究和的遗传改良提供了有价值的工具，这可能通过开发这种生物防治剂的改良菌株，从而更有效地控制入侵杂草。此外，我们开发的白眼突变菌株可能对该物种的其他转基因研究有用。
