Zhang Xiangrui, Bai Yu, Wang Shida, Shi Jun, Wu Haoxin
Key Laboratory of Integrative Biomedicine for Brain Diseases, School of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China.
National Famous Chinese Medicine Expert Inheritance Studio (Meng Jingchun), School of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Int J Mol Sci. 2025 May 12;26(10):4628. doi: 10.3390/ijms26104628.
Myasthenia gravis (MG) is an autoimmune di sease characterized by muscle weakness. Experimental autoimmune myasthenia gravis (EAMG) serves as an animal model for MG research. Despite advancements in EAMG modeling, limited drug absorption and variability in disease manifestation among animals resulted in a low success rate of model induction. This study aimed to optimize and standardize the modeling process by exploring different induction conditions to improve success rates. We employed female Lewis rats and C57BL/6 mice to compare their sensitivity to model induction and applied a controlled variable approach to acetylcholine receptor (AChR) and H37Ra dosage, mixing time, and injection sites. Results showed that Lewis rats were more suitable than C57BL/6 mice, and 75 µg AChR peptides were more effective than 50 µg. The immune-boosting effect of 1 mg H37Ra Mycobacterium tuberculosis was weaker than 2 mg. While drug mixing time had little impact, increasing injection sites on backs and including foot pads injection, significantly improved drug absorption.
重症肌无力（MG）是一种以肌肉无力为特征的自身免疫性疾病。实验性自身免疫性重症肌无力（EAMG）作为MG研究的动物模型。尽管EAMG建模取得了进展，但药物吸收有限以及动物之间疾病表现的变异性导致模型诱导成功率较低。本研究旨在通过探索不同的诱导条件来优化和标准化建模过程，以提高成功率。我们使用雌性Lewis大鼠和C57BL/6小鼠比较它们对模型诱导的敏感性，并对乙酰胆碱受体（AChR）和H37Ra剂量、混合时间和注射部位采用控制变量法。结果表明，Lewis大鼠比C57BL/6小鼠更适合，75μg AChR肽比50μg更有效。1mg结核分枝杆菌H37Ra的免疫增强作用弱于2mg。虽然药物混合时间影响不大，但增加背部注射部位并包括脚垫注射，可显著提高药物吸收。
