Kasimova Anastasia A, Arbatsky Nikolay P, Gornostal Ekaterina A, Shneider Mikhail M, Sheck Eugene A, Shashkov Alexander S, Shelenkov Andrey A, Mikhailova Yulia V, Azizov Ilya S, Edelstein Mikhail V, Perepelov Andrey V, Shpirt Anna M, Miroshnikov Konstantin A, Popova Anastasia V, Knirel Yuriy A
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia.
State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Russia.
Int J Mol Sci. 2025 May 15;26(10):4727. doi: 10.3390/ijms26104727.
is a significant nosocomial pathogen characterized by the ability to produce a wide variety of capsular polysaccharides (CPSs). The structures of a K102-type CPS isolated from KZ-1102 and its Smith degradation product were determined by sugar analysis, 1D and 2D H NMR spectroscopy, and C NMR spectroscopy. The K102 CPS biosynthesis gene cluster (KL102) contains genes for common sugar synthesis, K unit processing, capsule export, glycosyl transfer, initiating sugar phosphate transfer, and genes that encode d-GlcNAc/d-GalNAc dehydrogenase and phosphoglycerol transferase. The CPS is composed of a pentasaccharide repeating unit (K unit) consisting of a tetrasaccharide backbone including one α-d-Gal, three α-d-GlcNAc residues, and one residue of a β-d-Glc as a side chain. The tailspike depolymerase of the specific phage Cato was found to cleave the α-d-GlcNAc-(1→6)-α-d-GlcNAc linkage in the K102 CPS to give the monomer and dimer of the K repeating unit, which were characterized by high-resolution electrospray ionization mass spectrometry as well as H and C NMR spectroscopy.
是一种重要的医院病原体，其特点是能够产生多种荚膜多糖（CPSs）。通过糖分析、一维和二维氢核磁共振光谱以及碳核磁共振光谱，确定了从KZ - 1102分离出的K102型CPS及其史密斯降解产物的结构。K102 CPS生物合成基因簇（KL102）包含共同糖合成、K单位加工、荚膜输出、糖基转移、起始糖磷酸转移的基因，以及编码d - GlcNAc/d - GalNAc脱氢酶和磷酸甘油转移酶的基因。该CPS由一个五糖重复单元（K单位）组成，该重复单元由一个四糖主链组成，包括一个α - d - Gal、三个α - d - GlcNAc残基和一个作为侧链的β - d - Glc残基。发现特定噬菌体Cato的尾刺解聚酶可切割K102 CPS中的α - d - GlcNAc - (1→6)-α - d - GlcNAc键，生成K重复单元的单体和二聚体，通过高分辨率电喷雾电离质谱以及氢和碳核磁共振光谱对其进行了表征。
