The allelic diversity within genes controlling the vernalization requirement () and photoperiod response () determines the ability of wheat to adapt to a wide range of environmental conditions and influences grain yield. In this study, allelic variations at the , , and genes were studied for 89 accessions of from different eco-geographical regions of the world. The collection was evaluated for heading date in both field and greenhouse experiments under a long photoperiod and without vernalization. Based on heading date characteristics, 52 (58.4%) of the genotypes had a spring growth habit, and all of them carried at least one dominant allele, while 37 (41.6%) accessions had a winter growth habit and carried the triple recessive allele combination. The photoperiod-sensitive allele was detected in 85 (95.5%) accessions and the insensitive allele in four (4.5%) accessions. A total of 10 phenology gene profiles (haplotypes) were observed at four major genes in the germplasm collection. The LSD test revealed significant differences in the mean heading date among the different spring phenology gene profiles, both in greenhouse and field conditions. In addition, 21 microsatellite markers (simple sequence repeats, SSRs) were used to assess the genetic diversity in the collection. The 21 SSR markers amplified a total of 183 alleles across all the genotypes, with a mean of 3.2 alleles per locus. The polymorphic information content ranged from 0.49 to 0.94, with a mean of 0.84. The results of this study may be useful for both and common wheat breeding programs as a source of agronomic traits.