Ciszkowicz Ewa, Miłoś Anna, Łyskowski Andrzej, Buczkowicz Justyna, Nieczaj Anna, Lecka-Szlachta Katarzyna, Hus Konrad K, Sikora Karol, Neubauer Damian, Bauer Marta, Kamysz Wojciech, Bocian Aleksandra
Department of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszów University of Technology, al. Powstańców Warszawy 6, 35-959 Rzeszów, Poland.
Doctoral School of the Rzeszów University of Technology, al. Powstańców Warszawy 12, 35-959 Rzeszów, Poland.
Molecules. 2025 May 15;30(10):2167. doi: 10.3390/molecules30102167.
The treatment of proctological conditions, including hemorrhoids, anal fissures, and perianal abscesses, is often complicated by bacterial infections, particularly those involving multidrug-resistant . This study presents the synthesis, characterization, and biological evaluation of the newly designed synthetic peptide AMPEC4, inspired by cytotoxin 5 from snake venom. AMPEC4 demonstrated potent antimicrobial properties with MIC values of 100 and 200 µg/mL, effectively inhibiting biofilm formation (up to 84%) and eradicating the pre-formed biofilm by up to 35%. The antibacterial activity of AMPEC4 was further supported by a membrane permeabilization assay, demonstrating its capacity to disrupt bacterial membrane integrity in a dose-dependent manner. Furthermore, AMPEC4 significantly promoted fibroblast migration, a critical step in tissue regeneration, while exhibiting notable biocompatibility, as evidenced by the absence of hemolytic, cytotoxic, and genotoxic effects. By addressing both infection control and tissue regeneration, AMPEC4 represents a promising therapeutic strategy for managing chronic wounds, particularly in the challenging environment of the anorectal region. Its ability to target reference and clinical strains while accelerating the wound-healing process underscores its potential for future clinical applications.
直肠疾病的治疗，包括痔疮、肛裂和肛周脓肿，常常因细菌感染而复杂化，尤其是那些涉及多重耐药菌的感染。本研究介绍了受蛇毒细胞毒素5启发新设计的合成肽AMPEC4的合成、表征及生物学评价。AMPEC4表现出强大的抗菌特性，其最低抑菌浓度值为100和200 µg/mL，能有效抑制生物膜形成（高达84%）并消除高达35%的预先形成的生物膜。膜通透性测定进一步支持了AMPEC4的抗菌活性，表明其能够以剂量依赖方式破坏细菌膜完整性。此外，AMPEC4显著促进成纤维细胞迁移，这是组织再生中的关键步骤，同时表现出显著的生物相容性，溶血、细胞毒性和遗传毒性作用的缺失证明了这一点。通过解决感染控制和组织再生问题，AMPEC4代表了一种有前景的治疗策略，用于管理慢性伤口，尤其是在肛肠区域具有挑战性的环境中。它靶向参考菌株和临床菌株并加速伤口愈合过程的能力突出了其未来临床应用的潜力。
