Visualization of Single Polymer Chains with Atomic Force Microscopy: A Review.

Single-chain atomic force microscopy has emerged as a powerful and highly specialized technique, enabling the direct observation and analysis of various isolated polymer chains at the nano and micro scales. This work reviews the most relevant experimental cases utilizing this technique, aiming to shine light on the understanding of the physical appearance of freshly synthesized polymer chains, reveal unique chain conformations and related transitions, decipher the processes of polymer crystallization and self-assembly, study the mechanisms of polymer adsorption and desorption, observe the formation of single-chain nanoparticles, and explore many other related phenomena.