原子力显微镜成像在蛋白质中的应用综述。

Review on the applications of atomic force microscopy imaging in proteins.

机构信息

International Research Centre for Nano Handling and Manufacturing of China, Changchun University of Science and Technology, Changchun 130022, China; Ministry of Education Key Laboratory for Cross-Scale Micro and Nano Manufacturing, Changchun University of Science and Technology, Changchun 130022, China.

出版信息

Micron. 2022 Aug;159:103293. doi: 10.1016/j.micron.2022.103293. Epub 2022 May 13.

DOI:10.1016/j.micron.2022.103293

PMID:35636161

Abstract

Protein is an important component of human tissues and cells, which is closely related to various life activities. Therefore, studying the conformation of proteins can not only provide better analytic insight into its interactions with biomacromolecules, but also contribute to the biological and medical research. In recent years, the atomic force microscopy with excellent capacity shines brilliantly in obtaining information of biological samples, especially in the research of proteins. Herein, we highlight the advances and main contributions in the understanding of proteins conformation and its interactions with biomacromolecules by atomic force microscopy imaging technology. In addition, we have found that the relevant information of a single investigated object could be directly measured and obtained by atomic force microscopy, which avoids the indirectness and complexity of data analysis. Therefore, it is reasonable to believe that this work will be conducive to promoting the development of atomic force microscopy in the research field of protein imaging, and promoting its further applications in immunology, medicine and biology.

摘要

蛋白质是人体组织和细胞的重要组成部分，与各种生命活动密切相关。因此，研究蛋白质的构象不仅可以为其与生物大分子的相互作用提供更好的分析见解，还有助于生物和医学研究。近年来，原子力显微镜以其优异的能力在获取生物样本信息方面大放异彩，尤其是在蛋白质研究方面。本文重点介绍了原子力显微镜成像技术在理解蛋白质构象及其与生物大分子相互作用方面的进展和主要贡献。此外，我们发现原子力显微镜可以直接测量和获取单个研究对象的相关信息，避免了数据分析的间接性和复杂性。因此，可以合理地认为，这项工作将有助于推动原子力显微镜在蛋白质成像研究领域的发展，并促进其在免疫学、医学和生物学中的进一步应用。