Wells James E, Durso Lisa M, Ibekwe Abasiofiok M, Frye Jonathan G, Sharma Manan, Williams Clinton F, Shamimuzzaman Md
USDA Agricultural Research Service (ARS), U.S. Meat Animal Research Center, Meat Safety and Quality, Clay Center, NE 68933, USA.
USDA Agricultural Research Service (ARS), Agroecoystem Management Research, Lincoln, NE 68583, USA.
Pathogens. 2025 Apr 25;14(5):417. doi: 10.3390/pathogens14050417.
Bacteriological control strains with known characteristics ensure consistency and reproducibility of assay performance across different laboratories and are an important cornerstone of quality control in the microbiology laboratory. Ideally, control strains should be representative of the assay target and be widely available from reputable sources. However, for work involving antibiotic resistance most controls come from human and veterinary clinical sources and are not optimized for work in agriculturally impacted environments or not widely available. The objective of this work was to identify and make widely available two isolates sourced from agricultural production settings that could be used as external controls supporting method development, research and environmental monitoring for extended spectrum β-lactamase producing (ESBL) and tetracycline resistant . Previously collected suspects were screened based on antimicrobial susceptibility testing data, then confirmed as and characterized both phenotypically and genotypically. The positive control strain, ARS-C301 was ESBL positive and contained the CTX-M-55 and (A) genes, and the negative control strain, ARS-C101 was negative for both targets. Here we introduce two agriculturally sourced, fully characterized, and genetically sequenced control strains for use as laboratory controls in research involving extended-spectrum β-lactamase producing (ESBL) and tetracycline-resistant isolated from the environment, available via publicly accessible culture collections, and commercially as a quantitative pellet.
具有已知特性的细菌学对照菌株可确保不同实验室检测性能的一致性和可重复性，是微生物实验室质量控制的重要基石。理想情况下，对照菌株应代表检测目标，并且可从可靠来源广泛获得。然而，对于涉及抗生素耐药性的工作，大多数对照菌株来自人类和兽医临床来源，并未针对农业受影响环境中的工作进行优化，或者无法广泛获得。这项工作的目的是鉴定并广泛提供两株从农业生产环境中分离出的菌株，它们可用作外部对照，以支持超广谱β-内酰胺酶产生菌（ESBL）和四环素耐药菌的方法开发、研究及环境监测。根据抗菌药敏试验数据对先前收集的可疑菌株进行筛选，然后确认为[具体细菌名称未给出]，并进行表型和基因型特征分析。阳性对照菌株ARS-C301为ESBL阳性，含有CTX-M-55和[未明确的(A)基因]基因，阴性对照菌株ARS-C101对两个目标均为阴性。在此，我们引入两株源自农业、经过全面特征分析和基因测序的对照菌株，用于涉及从环境中分离出的超广谱β-内酰胺酶产生菌（ESBL）和四环素耐药菌的研究中的实验室对照，可通过公开的菌种保藏中心获得，也可作为定量菌珠进行商业购买。
