Alabí Córdova Amir Salvador, Pinho João Batista, Pereira Amanda Garcia, Galon Clémence, Ferreira Tiago Valadares, das Neves Lorena Freitas, de Oliveira Lopes Gabrielly, Machado Rosangela Zacarias, Moutailler Sara, André Marcos Rogério
Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences, Sao Paulo State University "Júlio de Mesquita Filho" (FCAV/UNESP), Jaboticabal 14884-900, Brazil.
Postgraduate Program in Ecology and Biodiversity Conservation, Federal University of Mato Grosso, UFMT, Cuiabá 78060-900, Brazil.
Pathogens. 2025 May 16;14(5):491. doi: 10.3390/pathogens14050491.
Despite numerous studies on haemosporidians in wild birds from Brazil, the presence of other vector-borne agents (VBA) such as spp., spp., and Onchocercidae filariids in avian hosts remains largely unknown. The low occurrence of these VBAs might be due to the low sensitivity of traditional molecular techniques. The microfluidic real-time PCR assay, known for its high sensitivity, has emerged as a promising method to detect and study the occurrence and diversity of VBAs in both arthropod vectors and vertebrate hosts. To validate previously and standardize newly designed microfluidic real-time PCR protocols, selected positive avian blood DNA samples for spp., spp., haemosporidians, and filariids were used. The molecular occurrence rates for the selected VBAs were 18.2% for spp., 0.36% for spp., 6.2% for spp., 4.7% for spp., and 6.5% for Onchocercidae filariids. The spp. B sequence detected in a clustered with , whereas the spp. sequence detected in a clustered with . While Onchocercidae filariid 1 sequences were detected in specimens of , and grouped with spp., one sequence detected in was ancestral to the clade comprising spp. and spp. High-throughput microfluidic real-time PCR assay can be used for screening VBAs in avian hosts from South America, but new primers/probe sets should be designed for VBA genotypes present in Brazil.
尽管对巴西野生鸟类中的血孢子虫进行了大量研究，但禽类宿主中其他媒介传播病原体（VBA）如 属、 属和盘尾丝虫科丝虫的存在情况仍 largely unknown。这些VBA的低发生率可能是由于传统分子技术的低敏感性。以高敏感性著称的微流控实时PCR检测法已成为一种有前途的方法，可用于检测和研究节肢动物媒介和脊椎动物宿主中VBA的发生情况和多样性。为了验证先前的微流控实时PCR方案并标准化新设计的方案，使用了针对 属、 属、血孢子虫和丝虫的选定阳性禽血DNA样本。选定VBA的分子发生率分别为： 属18.2%， 属0.36%， 属6.2%， 属4.7%，盘尾丝虫科丝虫6.5%。在一只 中检测到的 属B序列与 聚类，而在一只 中检测到的 属 序列与 聚类。虽然在 、 和 的标本中检测到了盘尾丝虫科丝虫1序列，且它们与 属聚类，但在 中检测到的一个序列是包括 属和 属的进化枝的祖先。高通量微流控实时PCR检测法可用于筛查南美禽类宿主中的VBA，但应针对巴西存在的VBA基因型设计新的引物/探针组。
