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调控玉米生长发育及菌根共生。

Regulates Maize Growth and Development and Mycorrhizal Symbiosis.

作者信息

Xie Kailing, Wang Guoqing, Ni Ying, Shi Minghui, Sun Lixue, Cheng Beijiu, Li Xiaoyu

机构信息

Key Laboratory of Crop Stress Resistance and High-Quality Biology of Anhui Province, Anhui Agricultural University, Hefei 230036, China.

出版信息

Plants (Basel). 2025 May 11;14(10):1438. doi: 10.3390/plants14101438.

Abstract

Hydroxyproline O-arabinosyltransferase (HPAT), a critical enzyme in plant glycosylation pathways, catalyzes the transfer of arabinose to the hydroxyl group of hydroxyproline residues. This enzyme contains a canonical GT95 glycosyltransferase, a structural hallmark of this carbohydrate-active enzyme family. HPAT mediates arabinosylation of diverse cellular targets, including cell wall extension and small signaling peptides. Emerging evidence has shown that HPAT orthologs regulate plant development and symbiotic interactions through post-translational modification of /LRR Extracellular (CLE) peptides. Although the molecular functions of genes have been characterized in model plants such as and , their roles remain unexplored in L. In this study, we used homozygous mutants to explore the function of the maize gene. Sequence analysis identified a N-terminal signal peptide targeting the Golgi apparatus and promoter elements responsive to AM fungal colonization. Phenotypic analysis revealed its negative regulatory role: promotes vegetative growth (increased plant height and accelerated flowering) and enhances AM symbiosis (increased colonization rate). Mechanistic studies demonstrated that possesses dual regulatory functions-the activation of auxin signaling and repression of -mediated arbuscular degradation pathways. In addition, overexpression of in inhibits growth (reduced plant height) and impairs symbiotic interactions. Our findings establish as a critical node to regulate auxin and symbiotic signaling, providing novel insights into plant glycosylation-mediated development. This work not only advances our understanding of maize growth regulation but also identifies potential targets for crop improvement through arabinosylation pathway manipulation.

摘要

羟脯氨酸O-阿拉伯糖基转移酶(HPAT)是植物糖基化途径中的一种关键酶,催化阿拉伯糖转移到羟脯氨酸残基的羟基上。该酶含有一个典型的GT95糖基转移酶,这是这个碳水化合物活性酶家族的一个结构特征。HPAT介导多种细胞靶点的阿拉伯糖基化,包括细胞壁延伸和小信号肽。新出现的证据表明,HPAT直系同源物通过对/LRR细胞外(CLE)肽的翻译后修饰来调节植物发育和共生相互作用。尽管在拟南芥和水稻等模式植物中已经对相关基因的分子功能进行了表征,但它们在玉米中的作用仍未得到探索。在本研究中,我们使用玉米纯合突变体来探索ZmHPAT基因的功能。序列分析确定了一个靶向高尔基体的N端信号肽和对丛枝菌根真菌定殖有反应的启动子元件。表型分析揭示了其负调控作用:ZmHPAT促进营养生长(增加株高和加速开花)并增强丛枝菌根共生(提高定殖率)。机制研究表明,ZmHPAT具有双重调控功能——激活生长素信号和抑制介导的丛枝降解途径。此外,在水稻中过表达ZmHPAT会抑制生长(降低株高)并损害共生相互作用。我们的研究结果确立了ZmHPAT作为调节生长素和共生信号的关键节点,为植物糖基化介导的发育提供了新的见解。这项工作不仅推进了我们对玉米生长调控的理解,还通过阿拉伯糖基化途径操作确定了作物改良的潜在靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ced/12115135/102d9b36417d/plants-14-01438-g001.jpg

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