Microbial Carbon Limitation Mediates Soil Organic Carbon Sequestration in Sugarcane-Watermelon Intercropping System.

Intercropping is an effective approach for enhancing soil organic carbon (SOC) sequestration. However, the effects of intercropping on SOC dynamics and the underlying factors in rhizosphere and bulk soils are still unclear. In this study, we examined the impacts of sugarcane monoculture and sugarcane-watermelon intercropping on soil properties, soil respiration, SOC fractions, and microbial C limitation with continuous two years in 2023-2024 years in the Nala area of Guangxi Province. Our results revealed that intercropping significantly decreased CO/SOC by 25% and microbial C limitation by 21% in the rhizosphere, with more pronounced reductions observed in bulk soil by 33% and 25%, respectively. This means that the intercropping reduced soil respiration and this effect can be offset by the rhizosphere effects. Additionally, the sugarcane-watermelon intercropping increased the contents of mineral-associated organic carbon (MAOC) by 1518% and particulate organic carbon (POC) by 3446%. The random forest analysis indicated that enzyme activities (explaining 20~38% of variation) and soil properties (explaining 22% of variation) were the primary drivers of reduced CO emissions. The PLS-PM showed that intercropping decreased microbial C limitation by influencing soil pH and soil water content (SWC), and then increased MAOC, which finally led to a decline in CO emissions. Overall, these findings highlight the decreasing CO emissions during the use of the intercropping system and the importance of microbial C limitation in the soil C cycle via soil respiration and SOC fractions.