Xing Yuwei, Liu Cong, Cui Xumeng, Lv Haonan, Wang Jun
Colleges of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao 266003, China.
Microorganisms. 2025 May 10;13(5):1103. doi: 10.3390/microorganisms13051103.
Presl is a rare hardy water lily at risk of extinction and has been included on the 'Red List' of threatened species of the International Union for the Conservation of Nature. To protect germplasm resources and propagate seedlings, this study conducted tissue culture and found that pathogenic endophytic fungal infection was the main reason for failure of tissue culture. Compared with the stems and leaves, the roots of had the highest rates of fungal infection during tissue culture. Subsequently, three isolated endophytic fungi, , , and sp., showed the highest frequency of occurrence in tissue culture. Furthermore, an antifungal formulation comprising 0.1 μg/mL pyrimidin suspension, 1 μg/mL mancozeb wettable powder, and 1 μg/mL carbendazim was constructed and could reduce the infection rates of root and stem tissues to 7.5% and 0%, respectively. Finally, the usefulness of this antifungal formulation for inhibition of endophytic fungi in tissue culture of was validated. This study not only provides important technical support for mass production of seedlings of , but also provides a scientific reference for the protection of endangered aquatic plant species.
普雷斯睡莲是一种珍稀的耐寒睡莲，面临灭绝风险，已被列入国际自然保护联盟濒危物种“红色名录”。为保护种质资源并培育幼苗，本研究进行了组织培养，发现致病性内生真菌感染是组织培养失败的主要原因。在组织培养过程中，与茎和叶相比，普雷斯睡莲的根真菌感染率最高。随后，三种分离出的内生真菌，即[具体真菌名称1]、[具体真菌名称2]和[具体真菌名称3] sp.，在组织培养中出现频率最高。此外，构建了一种由0.1μg/mL嘧啶悬浮液、1μg/mL代森锰锌可湿性粉剂和1μg/mL多菌灵组成的抗真菌配方，该配方可将根和茎组织的感染率分别降至7.5%和0%。最后，验证了这种抗真菌配方在普雷斯睡莲组织培养中抑制内生真菌的有效性。本研究不仅为普雷斯睡莲幼苗的大规模生产提供了重要技术支持，也为濒危水生植物物种的保护提供了科学参考。
