Laohavisuti Nongnuch, Boonchom Banjong, Rungrojchaipon Pesak, Boonmee Wimonmat, Seesanong Somkiat, Punthipayanon Sirichet
Department of Animal Production Technology and Fishery, School of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
Material Science for Environmental Sustainability Research Unit, School of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
Int J Mol Sci. 2025 Jul 30;26(15):7371. doi: 10.3390/ijms26157371.
A simple and rapid precipitation process was successfully employed to prepare silver phosphate (SP, AgPO). Two different phosphate sources: diammonium hydrogen phosphate ((NH)HPO) and dipotassium hydrogen phosphate (KHPO) were applied separately as the precursor, obtaining ((NH)HPO) and KHPO derived SP powders, named SP-A or SP-P, respectively. Fourier transform infrared (FTIR) spectra pointed out the vibrational characteristics of P-O and O-P-O interactions, confirming the presence of the PO43- functional group for SP. X-ray diffraction (XRD) patterns revealed that the SP crystallized in a cubic crystal structure. Whereas the field emission scanning electron microscope (FESEM) exposed spherical SP particles. The potentially antibacterial activity of SP-A and SP-P against bacterial , yeast , and fungal was subsequently investigated. All studied microorganisms were recovered and isolated from the aquatic plant during the tissue culture process. The preliminary result of the antimicrobial test revealed that SP-A has higher antimicrobial activity than SP-P. The superior antimicrobial efficiency of SP-A compared to SP-P may be attributed to its purity and crystallite size, which provide a higher surface area and more active sites. In addition, the presence of potassium-related impurities in SP-P could have negatively affected its antimicrobial performance. These findings suggest that SP holds potential as an antimicrobial agent for maintaining sterility in tissue cultures, particularly in aquatic plant systems. The growth of both . and . was suppressed effectively at 30 ppm SP-A, whereas 10 ppm of SP-A can suppress development. This present work also highlights the potential of SP at very low concentrations (10-30 ppm) for utilization as an effective antimicrobial agent in tissue culture, compared to a commercial antimicrobial agent, viz., acetic acid, at the same concentration.
采用一种简单快速的沉淀工艺成功制备了磷酸银（SP，AgPO）。分别使用两种不同的磷酸盐源：磷酸氢二铵（(NH₄)₂HPO₄）和磷酸氢二钾（KH₂PO₄）作为前驱体，得到分别命名为SP - A或SP - P的由(NH₄)₂HPO₄和KH₂PO₄衍生的SP粉末。傅里叶变换红外（FTIR）光谱指出了P - O和O - P - O相互作用的振动特征，证实了SP中PO₄³⁻官能团的存在。X射线衍射（XRD）图谱表明SP结晶为立方晶体结构。而场发射扫描电子显微镜（FESEM）显示出球形的SP颗粒。随后研究了SP - A和SP - P对细菌、酵母和真菌的潜在抗菌活性。所有研究的微生物均在组织培养过程中从水生植物中回收和分离得到。抗菌测试的初步结果表明，SP - A比SP - P具有更高的抗菌活性。与SP - P相比，SP - A卓越的抗菌效率可能归因于其纯度和微晶尺寸，这提供了更高的表面积和更多的活性位点。此外，SP - P中钾相关杂质的存在可能对其抗菌性能产生了负面影响。这些发现表明，SP作为一种抗菌剂在维持组织培养中的无菌状态方面具有潜力，特别是在水生植物系统中。在30 ppm的SP - A下，细菌和酵母的生长均受到有效抑制，而10 ppm的SP - A就能抑制真菌的生长。与相同浓度的商业抗菌剂即乙酸相比，本研究还突出了极低浓度（10 - 30 ppm）的SP在组织培养中作为有效抗菌剂的潜力。
