Murthy Hosakatte Niranjana, Joseph Kadanthottu Sebastian, Paek Kee Yoeup, Park So Young
Department of Botany, Karnatak University, Dharwad, India.
Department of Horticultural Science, Chungbuk National University, Cheongju, Republic of Korea.
Front Plant Sci. 2023 Apr 19;14:1159588. doi: 10.3389/fpls.2023.1159588. eCollection 2023.
Plant micropropagation has been adapted in the fields of agriculture, horticulture, forestry, and other related fields for large-scale production of elite plants. The use of liquid media and adoption of bioreactors have escalated the production of healthy plants. Several liquid-phase, gas-phase, temporary immersion, and other modified bioreactors have been used for plant propagation. The design, principle, operational mode, merits, and demerits of various bioreactors used for the regeneration of propagules, such as bulblets, cormlets, rhizomes, microtubers, shoots (subsequent rooting), and somatic embryos, are discussed here. In addition, various parameters that affect plant regeneration are discussed with suitable examples.
植物微繁殖已在农业、园艺、林业及其他相关领域得到应用,用于大规模生产优良植株。液体培养基的使用和生物反应器的采用提高了健康植株的产量。几种液相、气相、临时浸没及其他改良生物反应器已用于植物繁殖。本文讨论了用于繁殖体(如小鳞茎、小种球、根茎、微型块茎、芽(后续生根)和体细胞胚)再生的各种生物反应器的设计、原理、操作模式、优点和缺点。此外,还结合适当的例子讨论了影响植物再生的各种参数。