Ji Yaru, Ali Munwar, Xu Chang, Wang Jia, Kulyar Md F, Nawaz Shah, Mehmood Khalid, Liu Mingming, Li Kun
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Vet Sci. 2025 May 20;12(5):504. doi: 10.3390/vetsci12050504.
The yak (), prevalent at an altitude between 3000 and 5000 m above sea level, provides the local inhabitants with meat, milk, leather, fuel (dung), and transport. However, intestinal zoonotic parasites seriously endanger its holistic well-being. The prime concern of this study is to investigate the prevalence of four globally ubiquitous zoonotic enteric protozoans, namely spp., , spp., and in yaks from different areas of Lhasa, Xizang. In the given study, 377 yak fecal samples from various regions in Lhasa were obtained, including 161 samples from Linzhou County, 66 samples from Dangxiong County, and 150 samples from the Nimu County cattle farms. Molecular identification of these protozoans was done after amplification using PCR and sequencing of PCR-positive samples, and further phylogenetic analysis was performed. The results indicated that the prevalence of spp., , , and spp. in yak farms in Linzhou County was 48.5, 22.9, 47.8, and 90.7%; 65.2, 13.6, 72.7, and 87.9% in Dangxiong County; and 56.0, 29.3, 58.0, and 80.0%, respectively, in Nimu County. The results of this study provide a basic reference for preventing and controlling intestinal parasites in yaks in Lhasa, Xizang.
牦牛分布于海拔3000至5000米的地区，为当地居民提供肉、奶、皮革、燃料（粪便）和运输工具。然而，肠道人畜共患寄生虫严重威胁着牦牛的整体健康。本研究的主要关注点是调查四种全球普遍存在的人畜共患肠道原生动物，即等孢球虫属、隐孢子虫属、贾第虫属和结肠小袋纤毛虫在西藏拉萨不同地区牦牛中的感染率。在本研究中，采集了拉萨市不同地区的377份牦牛粪便样本，其中包括林周县的161份样本、当雄县的66份样本和尼木县养殖场的150份样本。通过PCR扩增和对PCR阳性样本进行测序后，对这些原生动物进行分子鉴定，并进一步进行系统发育分析。结果表明，林周县牦牛养殖场中，等孢球虫属、隐孢子虫属、贾第虫属和结肠小袋纤毛虫的感染率分别为48.5%、22.9%、47.8%和90.7%；当雄县分别为65.2%、13.6%、72.7%和87.9%；尼木县分别为56.0%、29.3%、58.0%和80.0%。本研究结果为西藏拉萨牦牛肠道寄生虫的防控提供了基础参考。
