Ebbe S, Adrados C, Phalen E
Exp Hematol. 1985 Sep;13(8):817-20.
Megakaryocytopoiesis was evaluated in long-term cultures of normal murine marrow to determine whether the number and size of megakaryocytes were independent or interdependent. Numbers of megakaryocytes and granulocytes varied widely in different experiments, due, in part, to varying concentrations of hydrocortisone in the culture medium. The sizes of acetylcholinesterase-positive cells were the same in cultures with as much as a 20-fold difference in megakaryocyte numbers. These results indicate that, in the closed culture system containing normal stromal cells, megakaryocyte size and number are not reciprocal as they were in many previously reported cultures of S1/S1d mouse marrow. The results suggest that separate stromal functions may determine precursor cell proliferation and nuclear endomitosis in megakaryocytes in vitro. The relationship of these findings to regulation of megakaryocytopoiesis in vivo remains speculative.
在正常小鼠骨髓的长期培养中评估巨核细胞生成，以确定巨核细胞的数量和大小是独立的还是相互依存的。在不同实验中，巨核细胞和粒细胞的数量差异很大，部分原因是培养基中氢化可的松的浓度不同。在巨核细胞数量相差多达20倍的培养物中，乙酰胆碱酯酶阳性细胞的大小相同。这些结果表明，在含有正常基质细胞的封闭培养系统中，巨核细胞的大小和数量并不像许多先前报道的S1/S1d小鼠骨髓培养物那样呈反比关系。结果表明，不同的基质功能可能决定体外巨核细胞中前体细胞的增殖和核内有丝分裂。这些发现与体内巨核细胞生成调节的关系仍具有推测性。
