Zucali J R, Sulkowski E
Exp Hematol. 1985 Sep;13(8):833-7.
Erythropoietin (Epo) has been isolated from raw human urine of anemic patients by controlled-pore glass and silicic acid chromatography. A purification factor of not less than 100-fold and recovery of about 80% of activity was routinely achieved. The resulting Epo preparation is active both in vivo and in vitro and can be neutralized by an antibody to human Epo indicating the native state and identity of the isolated Epo molecule. The preparation is stable at near neutral pH and is amenable to further purification.
促红细胞生成素（Epo）已通过可控孔径玻璃和硅酸色谱法从贫血患者的原始人尿中分离出来。常规可实现不低于100倍的纯化因子和约80%的活性回收率。所得的Epo制剂在体内和体外均具有活性，并且可以被抗人Epo抗体中和，这表明分离出的Epo分子的天然状态和同一性。该制剂在接近中性的pH值下稳定，并且适合进一步纯化。
