Cutler R L, Johnson G R, Nicola N A
Exp Hematol. 1985 Oct;13(9):899-905.
Although erythropoietins from human and sheep sources have been purified, a mouse erythropoietin remains essentially uncharacterized despite the fact that the mouse is most commonly used to assay for erythropoietin. Because other murine growth factors may have erythropoietic activity it will be essential to compare these with erythropoietin from murine sources rather than from other species. Here we compare the physicochemical properties and some of the bioactivities of erythropoietins from anemic mouse serum and human urine in parallel fractionations. Both molecules showed similar molecular weights (40-45,000) by gel filtration and similar retention characteristics on reverse-phase high-performance columns. However, they differed in their relative solubility in ammonium sulfate, hydrophobicity on phenyl-Sepharose, and in their ability to bind to a phenyl-boronate agarose column. Despite these differences the two molecules stimulated the same number of CFU-E in the mouse fetal liver assay and did so with similar dose-response relationships. The data indicate that putative murine erythropoietic stimuli must be compared with murine erythropoietin and not human urine erythropoietin before concluding, on the basis of fractionation results, that a factor is different from erythropoietin.
尽管来自人和羊源的促红细胞生成素已被纯化，但小鼠促红细胞生成素基本上仍未得到充分表征，尽管小鼠是最常用于检测促红细胞生成素的动物。由于其他鼠类生长因子可能具有促红细胞生成活性，因此将它们与鼠源而非其他物种来源的促红细胞生成素进行比较至关重要。在此，我们通过平行分级分离比较了贫血小鼠血清和人尿中促红细胞生成素的物理化学性质和一些生物活性。通过凝胶过滤，两种分子均显示出相似的分子量（40 - 45,000），并且在反相高效柱上具有相似的保留特性。然而，它们在硫酸铵中的相对溶解度、在苯基 - 琼脂糖上的疏水性以及与苯基硼酸琼脂糖柱结合的能力方面存在差异。尽管存在这些差异，但在小鼠胎儿肝脏检测中，这两种分子刺激相同数量的红细胞集落形成单位 - 红细胞（CFU - E），并且剂量 - 反应关系相似。数据表明，在根据分级分离结果得出某一因子不同于促红细胞生成素的结论之前，必须将假定的鼠类促红细胞生成刺激物与鼠源促红细胞生成素而非人尿促红细胞生成素进行比较。
