Randall G, Jacobs P
Exp Hematol. 1985 Oct;13(9):874-8.
Cyclosporin-A levels were determined by radioimmunoassay in plasma or whole blood, using split samples collected from patients receiving this agent as the only form of immunosuppression following allogeneic bone marrow transplantation. In the plasma assay the temperature at which centrifugation took place was critical since the mean levels were approximately 30% higher with separation at 37 degrees C in comparison to 20 degrees C or lower. Furthermore, the level in whole blood samples was 2.4 times higher than that from the matching serum. In addition, anticoagulated blood that had been frozen and then thawed was technically more difficult to pipette and resulted in a recovery of only 83% of the cyclosporin when compared with assay using fresh blood. In contrast, consistent measurements were obtained either when whole blood was stored at 4 degrees C and then well mixed and diluted in buffer immediately prior to use or when such buffered samples were frozen and thawed immediately before analysis. The latter modifications render the whole blood assay a practical and reliable means for monitoring cyclosporin-A concentrations and may avoid excessive and the potentially nephrotoxic levels achieved when plasma levels are held in ranges previously considered therapeutic.
采用放射免疫分析法测定接受异基因骨髓移植后仅接受环孢素A作为免疫抑制治疗的患者血浆或全血中环孢素A的水平。在血浆检测中，离心温度至关重要，因为与20℃或更低温度下分离相比，37℃分离时的平均水平约高30%。此外，全血样本中的水平比匹配血清中的水平高2.4倍。另外，冷冻后再解冻的抗凝血液在技术上更难移液，与使用新鲜血液检测相比，环孢素的回收率仅为83%。相比之下，当全血在4℃储存，然后在使用前立即充分混合并在缓冲液中稀释时，或者当这种缓冲样本在分析前立即冷冻和解冻时，均可获得一致的测量结果。后一种改进使全血检测成为监测环孢素A浓度的实用且可靠的方法，并且可以避免在血浆水平维持在先前认为具有治疗作用的范围内时出现过高且可能具有肾毒性的水平。
