Choppin J, Egrié J, Casadevall N, Lacombe C, Muller O, Varet B
Exp Hematol. 1987 Feb;15(2):171-6.
The hydrophobicity, ionic charges, degrees of glycosylation, and Western blot patterns of murine plasma erythropoietin and erythropoietin produced by clones of two murine erythroleukemic cell lines (IW32 and NN10) were compared. Erythropoietins from these different sources exhibited a number of similarities in their biochemical properties: identical retention on DEAE Affigel blue column and similar apparent molecular weights (30-36 kDa) in Western blot analysis. However, differences were also observed: heterogeneous binding to different lectin columns and varied retention on a phenyl Sepharose column. The data thus confirm that these murine plasma erythropoietins have biochemical properties in common with previously studied erythropoietins from humans or sheep. The major difference between erythropoietins secreted by erythroleukemic cell lines and other molecules belonging to the same family appears to be related to their glycosylation.
比较了小鼠血浆促红细胞生成素以及两种小鼠红白血病细胞系（IW32和NN10）克隆产生的促红细胞生成素的疏水性、离子电荷、糖基化程度和蛋白质免疫印迹模式。来自这些不同来源的促红细胞生成素在生化特性上表现出许多相似之处：在DEAE琼脂糖蓝柱上保留相同，在蛋白质免疫印迹分析中表观分子量相似（30 - 36 kDa）。然而，也观察到了差异：与不同凝集素柱的异质结合以及在苯基琼脂糖柱上的不同保留情况。这些数据因此证实，这些小鼠血浆促红细胞生成素具有与先前研究的人或羊促红细胞生成素相同的生化特性。红白血病细胞系分泌的促红细胞生成素与同一家族的其他分子之间的主要差异似乎与其糖基化有关。
