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一种用于多胺等度高效液相色谱分析的简化方法。

A simplified method for isocratic HPLC analysis of polyamines.

作者信息

Whitmore W L, Slotkin T A

出版信息

Experientia. 1985 Sep 15;41(9):1209-11. doi: 10.1007/BF01951733.

DOI:10.1007/BF01951733
PMID:4043337
Abstract

A simple technique is described for the separation and analysis of polyamines in tissues and body fluids, utilizing precolumn clean-up on disposable CM-cellulose columns, followed by an automatable HPLC procedure. Complete separation and analysis takes 12-15 min per sample with sensitivity in the pmole range.

摘要

本文描述了一种用于分离和分析组织及体液中多胺的简单技术，该技术利用一次性CM - 纤维素柱进行柱前净化，随后采用可自动化的高效液相色谱法。每个样品的完全分离和分析耗时12 - 15分钟，灵敏度在皮摩尔范围内。

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A simplified method for isocratic HPLC analysis of polyamines.一种用于多胺等度高效液相色谱分析的简化方法。
Experientia. 1985 Sep 15;41(9):1209-11. doi: 10.1007/BF01951733.
2
Chromophoric determination of putrescine, spermidine and spermine with dabsyl chloride by high-performance liquid chromatography and thin-layer chromatography.采用丹磺酰氯通过高效液相色谱法和薄层色谱法对腐胺、亚精胺和精胺进行发色测定。
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A dansyl chloride-HPLC method for the determination of polyamines.一种用于测定多胺的丹磺酰氯-高效液相色谱法。
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引用本文的文献

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Cerebral ischemia enhances polyamine oxidation: identification of enzymatically formed 3-aminopropanal as an endogenous mediator of neuronal and glial cell death.脑缺血增强多胺氧化：鉴定酶促形成的3-氨基丙醛为神经元和神经胶质细胞死亡的内源性介质。
J Exp Med. 1998 Jul 20;188(2):327-40. doi: 10.1084/jem.188.2.327.

本文引用的文献

1
Critical periods for the role of ornithine decarboxylase and the polyamines in growth and development of the rat: Effects of exposure to α-difluoromethylornithine during discrete prenatal or postnatal intervals.鸟氨酸脱羧酶和多胺在大鼠生长发育中作用的关键时期：在产前或产后不同时间段暴露于α-二氟甲基鸟氨酸的影响
Int J Dev Neurosci. 1983;1(2):113-27. doi: 10.1016/0736-5748(83)90038-2.
2
High performance liquid chromatographic separation and fluorescence detection of polyamines in plasma and erythrocytes.血浆和红细胞中多胺的高效液相色谱分离及荧光检测
Ann Clin Lab Sci. 1980 Mar-Apr;10(2):128-36.
3
High-performance liquid chromatographic procedure for the simultaneous determination of the natural polyamines and their monoacetyl derivatives.
同时测定天然多胺及其单乙酰衍生物的高效液相色谱法。
J Chromatogr. 1980 Dec 12;221(2):227-35. doi: 10.1016/s0378-4347(00)84307-8.
4
Simple method for the simultaneous determination of histamine, polyamines and histone H1.同时测定组胺、多胺和组蛋白H1的简便方法。
J Chromatogr. 1981 Jan 23;205(1):155-64. doi: 10.1016/s0021-9673(00)81823-3.
5
Ornithine decarboxylase and polyamines in tissues of the neonatal rat: effects of alpha-difluoromethylornithine, a specific, irreversible inhibitor of ornithine decarboxylase.新生大鼠组织中的鸟氨酸脱羧酶和多胺：鸟氨酸脱羧酶特异性不可逆抑制剂α-二氟甲基鸟氨酸的作用
J Pharmacol Exp Ther. 1982 Sep;222(3):741-5.
6
Femtomolar ion-pair high-performance liquid chromatographic method for determining Dns-polyamine derivatives of red blood cell extracts utilizing an automated polyamine analyzer.利用自动多胺分析仪，采用飞摩尔离子对高效液相色谱法测定红细胞提取物的丹磺酰多胺衍生物。
J Chromatogr. 1982 Aug 6;245(1):101-8. doi: 10.1016/s0021-9673(00)82479-6.
7
Polyamines in brain and heart of the neonatal rat: effects of inhibitors of ornithine decarboxylase and spermidine synthase.新生大鼠脑和心脏中的多胺：鸟氨酸脱羧酶和亚精胺合酶抑制剂的作用。
Life Sci. 1984 Sep 3;35(10):1125-31. doi: 10.1016/0024-3205(84)90078-x.
8
Ornithine decarboxylase as a biological and pharmacological tool.鸟氨酸脱羧酶作为一种生物学和药理学工具。
Pharmacology. 1980;20(3):117-29. doi: 10.1159/000137355.
9
Development of polyamine and biogenic amine systems in brains and hearts of neonatal rats given dexamethasone: role of biochemical alterations in cellular maturation for producing deficits in ontogeny of neurotransmitter levels, uptake, storage and turnover.给予地塞米松的新生大鼠脑和心脏中多胺和生物胺系统的发育：生化改变在细胞成熟中对神经递质水平、摄取、储存和周转个体发生缺陷产生的作用。
J Pharmacol Exp Ther. 1982 Jun;221(3):686-93.
10
An automated micromethod for the quantitative analysis of di- and polyamines utilizing a sensitive high pressure liquid chromatographic procedure.一种利用灵敏的高压液相色谱法对二胺和多胺进行定量分析的自动化微量方法。
FEBS Lett. 1974 Apr 15;41(1):99-103. doi: 10.1016/0014-5793(74)80963-4.