不同根管封闭剂的磷灰石形成能力（一项对比研究）

Apatite-Forming Ability of Different Root Canal Sealers (A Comparative Study).

作者信息

Al-Askary Raghad A, Al-Ashou Wiaam M O, Al-Jubori Sawsan H

机构信息

Department of Conservative Dentistry, College of Dentistry, University of Mosul, Mosul, Iraq.

出版信息

J Int Soc Prev Community Dent. 2025 Apr 30;15(2):173-183. doi: 10.4103/jispcd.jispcd_12_25. eCollection 2025 Mar-Apr.

DOI:10.4103/jispcd.jispcd_12_25

PMID:40433434

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12105809/

Abstract

AIM

Hydroxyapatite (HA) formation ability of endodontic sealers is considered an index of their bioactivity and plays a significant role in hard-tissue inductivity and elevating the sealing ability of the root canal system. This study evaluated and compared the apatite-forming ability of endodontic sealers after immersion in phosphate buffer saline (PBS).

METHODS

Eighty sealer specimens, 20 samples for each sealer (GuttaFlow2, GuttaFlow Bioseal, ProRoot mineral trioxide aggregate [MTA], and MTA Fillapex), measuring 1.0 ± 0.1 mm in thickness and 20 ± 0.1 mm in diameter, were prepared, then incubated at 37°C and 95% relative humidity for three times their setting time to ensure complete setting. The disc samples were de-molded, and 10 sealer discs of each sealer were immersed for 28 days in PBS, while the other discs were not immersed. Morphological and elemental analyses for the surface precipitate of each sealer's disc were analyzed with a field emission scanning electron microscope with energy-dispersive X-ray spectroscopy (FESEM). In addition, X-ray diffraction (XRD) and Raman spectroscopy analyses were used to assess the HA precipitate.

RESULTS

The FESEM revealed a superficial layer of apatite spherulites coated on (GuttaFlow Bioseal, ProRoot MTA, and MTA Fillapex) sealers after 28 days of immersion in PBS. The calcium to phosphorus (Ca/P) ratio of the coating indicated the formation of HA deposits which is higher for ProRoot MTA followed by MTA Fillapex and then by GuttaFlow Bioseal. Apatite deposits were confirmed by XRD through their characteristic peaks and Raman spectra through their band at 960 cm.

CONCLUSIONS

This research approves the excellent bioactivity of ProRoot MTA. In addition, both MTA Fillapex and GuttaFlow Bioseal sealer have bioactive ability.

摘要

目的

牙髓封闭剂的羟基磷灰石（HA）形成能力被视为其生物活性指标，在硬组织诱导性和提高根管系统封闭能力方面发挥着重要作用。本研究评估并比较了牙髓封闭剂在磷酸盐缓冲盐水（PBS）中浸泡后的磷灰石形成能力。

方法

制备了80个封闭剂样本，每种封闭剂（GuttaFlow2、GuttaFlow Bioseal、ProRoot矿物三氧化物凝聚体[MTA]和MTA Fillapex）20个样本，厚度为1.0±0.1毫米，直径为20±0.1毫米，然后在37°C和95%相对湿度下孵育三倍于其凝固时间以确保完全凝固。将圆盘样本脱模，每种封闭剂的10个封闭剂圆盘在PBS中浸泡28天，而其他圆盘不浸泡。使用带有能量色散X射线光谱仪（FESEM）的场发射扫描电子显微镜对每个封闭剂圆盘的表面沉淀物进行形态学和元素分析。此外，还使用X射线衍射（XRD）和拉曼光谱分析来评估HA沉淀物。

结果

FESEM显示，在PBS中浸泡28天后，（GuttaFlow Bioseal、ProRoot MTA和MTA Fillapex）封闭剂表面有一层磷灰石球晶覆盖。涂层的钙磷（Ca/P）比表明形成了HA沉积物，ProRoot MTA的HA沉积物最高，其次是MTA Fillapex，然后是GuttaFlow Bioseal。通过XRD的特征峰和拉曼光谱在960 cm处的谱带证实了磷灰石沉积物的存在。