Banyal Aditya, Thakur Rahul, Thakur Pryanka, Thakur Vikram, Chand Duni, Kumar Pradeep
Faculty of Applied Sciences and Biotechnology, Shoolini University of Biotechnology and Management Sciences, Solan, HP 173229 India.
Department of Biosciences and Technology, Maharishi Markandeshwar Engineering College, Maharishi Markandeshwar University, Mullana, Ambala, Haryana 133203 India.
3 Biotech. 2025 Jun;15(6):187. doi: 10.1007/s13205-025-04351-0. Epub 2025 May 26.
Vinblastine, a potent anticancer drug, is traditionally extracted from , but its low yield and high production costs necessitate alternative sources. In this study, twelve endophytic fungi were isolated from collected in Himachal Pradesh, India. Among these, isolate VPF-2, identified as through morphological and molecular analysis, demonstrated significant vinblastine production (8.673 mg/L) in M3 medium broth. PCR screening confirmed the presence of key biosynthetic genes, desacetoxyvindoline-4-hydroxylase (D4H) and tryptophan decarboxylase (TDC), in VPF-2. The fungal-derived vinblastine exhibited cytotoxic activity against MDCK cancer cells, with an IC value of 69.03 µg/mL. Structural characterization using HPTLC, UV, FTIR, LC-ESI-MS/MS, and NMR confirmed the compound's identity. This study reports the highest vinblastine yield from to date, highlighting its potential as a sustainable and scalable alternative for industrial vinblastine production.
长春碱是一种强效抗癌药物，传统上是从[具体植物名称缺失]中提取的，但由于其产量低和生产成本高，需要寻找替代来源。在本研究中，从印度喜马偕尔邦采集的[具体植物名称缺失]中分离出12种内生真菌。其中，通过形态学和分子分析鉴定为[具体真菌名称缺失]的分离株VPF-2，在M3培养基肉汤中显示出显著的长春碱产量（8.673毫克/升）。PCR筛选证实VPF-2中存在关键生物合成基因，去乙酰氧基长春花碱-4-羟化酶（D4H）和色氨酸脱羧酶（TDC）。真菌来源的长春碱对MDCK癌细胞表现出细胞毒性活性，IC值为69.03微克/毫升。使用HPTLC、UV、FTIR、LC-ESI-MS/MS和NMR进行的结构表征证实了该化合物的身份。本研究报道了迄今为止[具体植物名称缺失]中长春碱的最高产量，突出了其作为工业长春碱生产可持续和可扩展替代方案的潜力。
