Gregoraszczuk E
Folia Histochem Cytobiol. 1985;23(1-2):11-6.
Primary cell cultures of bovine corpora lutea were used in order to examine their morphology and secretion of progesterone and androgen in vitro. The cells were grown as monolayers up to 6 days at 37 degrees C medium 199 supplemented with 10% calf serum. The concentration of progesterone and androgen was measured using appropriate radioimmunoassays [1,3] respectively. Luteal cells were cultured with addition of the following amounts of hormones: 100 ng LH, 10 i.u. hCG, 100 ng PRL, 150 ng Estradiol 17 beta and 150 ng Testosterone/ml of culture medium. The luteal cells also created considerable amounts of androgens. It was found that only estradiol added to the culture medium caused an increase in the level of testosterone. Progesterone secretion following the addition of hormones increased under the influence of LH, T, and E2 in statistically significant manner while hCG and PRL had no statistically significant effects.
为了在体外检测牛黄体的形态以及孕酮和雄激素的分泌情况，使用了牛黄体的原代细胞培养物。细胞在补充有10%小牛血清的199培养基中，于37℃以单层培养长达6天。分别使用适当的放射免疫分析法[1,3]测量孕酮和雄激素的浓度。用以下量的激素培养黄体细胞：每毫升培养基中加入100 ng促黄体生成素（LH）、10国际单位人绒毛膜促性腺激素（hCG）、100 ng催乳素（PRL）、150 ng 17β-雌二醇和150 ng睾酮。黄体细胞也产生了相当数量的雄激素。发现仅添加到培养基中的雌二醇会导致睾酮水平升高。添加激素后，孕酮分泌在LH、睾酮（T）和雌二醇（E2）的影响下以统计学显著方式增加，而hCG和PRL没有统计学显著影响。
