Gregoraszczuk E L, Dudek D, Wojtusiak A
Department of Physiology, Jagiellonian University, Krakow, Poland.
Endocrinol Exp. 1990 Dec;24(4):465-70.
Corpora lutea removed from ovarian of cycling pigs in early luteal phase (1-3 days) were used. After enzyme dispersion the luteal cells were suspended in medium M 199 supplemented with 10% of calf serum. Cultures were carried out in triplicate and incubated at 37 degrees C for 24 h. After that time the following hormones were added to the culture medium; 100 ng LH, or 100 ng PRL or 100 ng LH plus 100 ng PRL. The cells were incubated with hormone for 6, 12, 24 and 48 h. LH added to the medium resulted in the increase of progesterone secretion (P less than 0.05) only during 6 h incubation (140% of control progesterone). Stimulatory effect of PRL was observed only after 12 h incubation (300% of control progesterone; P less than 0.001). The addition of LH plus PRL decreased progesterone secretion after 6 h incubation (33.3% of control progesterone; P less than 0.05), a little stimulatory effect being observed after 24 h incubation (141% of control progesterone). This study confirmed the results obtained with monolayer cultures previously described. PRL appeared to be a luteotrophic hormone which is responsible for the increase of progesterone secretion by the early developing corpus luteum of pig. From these data it was concluded that luteal cells may require either LH or prolactin but not both at a time to sustain higher level of progesterone secretion.
使用从处于黄体早期（1 - 3天）的发情期猪卵巢中取出的黄体。酶消化后，黄体细胞悬浮于添加了10%小牛血清的M199培养基中。培养一式三份进行，并在37℃孵育24小时。之后，向培养基中添加以下激素：100 ng促黄体生成素（LH），或100 ng催乳素（PRL），或100 ng LH加100 ng PRL。细胞与激素一起孵育6、12、24和48小时。添加到培养基中的LH仅在孵育6小时期间导致孕酮分泌增加（P < 0.05）（为对照孕酮的140%）。仅在孵育12小时后观察到PRL的刺激作用（为对照孕酮的300%；P < 0.001）。添加LH加PRL在孵育6小时后降低了孕酮分泌（为对照孕酮的33.3%；P < 0.05）,在孵育24小时后观察到轻微的刺激作用（为对照孕酮的141%）。本研究证实了先前所述单层培养获得的结果。PRL似乎是一种黄体营养激素，它负责猪早期发育的黄体分泌孕酮的增加。从这些数据得出结论，黄体细胞可能一次只需要LH或催乳素中的一种，而不是两者都需要来维持较高水平的孕酮分泌。
