Pietrzak M, Kozik M B
Folia Histochem Cytobiol. 1985;23(1-2):27-32.
The purpose of this investigation was to evaluate the influence of the antimitotic drug CCNU on the morphology of the hypothalamo-hypophyseal neurosecretory system of rat. Adult Wistar rats were treated intragastrically with 2.5 mg CCNU once a week during 3 consecutive weeks and 5 mg at the end of the 4th week. The brains and hypophyses were fixed in Zenker-formol solution. Paraffin slices were stained with chromhematoxylin to demonstrate neurosecretory material and with cresyl violet. PAS reaction was also performed. The experiment resulted in disturbances of the neurosecretory function of the hypothalamo-hypophyseal system in form of alteration in the content of neurosecretion in the neuronal cytoplasm and processes within supraoptic and paraventricular nuclei as well as in the neurohypophysis. The morphometric measurements showed enlargement of the cell nuclei and cytoplasm volumes in the nucleus supraopticus of hypothalamus.
本研究的目的是评估抗有丝分裂药物环己亚硝脲（CCNU）对大鼠下丘脑 - 垂体神经分泌系统形态的影响。成年Wistar大鼠连续3周每周一次经胃给予2.5 mg CCNU，在第4周结束时给予5 mg。将大脑和垂体固定于Zenker - 甲醛溶液中。石蜡切片用苏木精染色以显示神经分泌物质，并用甲酚紫染色。还进行了PAS反应。实验导致下丘脑 - 垂体系统神经分泌功能紊乱，表现为视上核和室旁核以及神经垂体中神经元细胞质和突起内神经分泌含量的改变。形态计量学测量显示下丘脑视上核中细胞核和细胞质体积增大。
