Wang Linjie, Wang Lu, Jing Lin, Yang Yang, Miao Hui, Liu Xiaoya, Suonanmu Dongzhi, Ren Qingjia, Wang Fei, Li Caolong
Department of Chemistry, School of Science, China Pharmaceutical University, Nanjing, 211198, Jiangsu, People's Republic of China.
Anhui Provincial Joint Key Laboratory for Innovative Drug Research and Industry Integration, School of Chemistry and Materials Engineering, Fuyang Normal University, Fuyang, 236037, People's Republic of China.
Mikrochim Acta. 2025 May 28;192(6):380. doi: 10.1007/s00604-025-07248-0.
A ratio fluorescence/colorimetry/smartphone triple-mode sensor based on Ru-CN was established for precise and rapid detection of melatonin (MT). Specifically, Ru-CN with fine luminous characteristic and peroxidase-like activity catalyzes the oxidation of OPD to generate luminescent DAP which in turn suppresses intrinsic fluorescence of Ru-CN because of the fluorescence inner filter effect (IFE). MT can inhibit this catalytic process, leading to partial recovery of the fluorescence of Ru-CN and the decrease in UV-vis signal of the reaction system, as well as the gradual fading of the yellow color of the reaction solution. Hence, this triple-mode sensor can realize precise, reliable, and high-throughput determination of MT with correlative detection limits of 0.32, 0.46, and 0.67 μM. Moreover, this triple-mode sensor has been applied to MT detection in multiple juice samples with recoveries ranging from 86.00 to 99.68%, which indicates the suitability of the novel idea for the research and development of multimode optical sensors for food analysis.
基于Ru-CN构建了一种用于精确快速检测褪黑素（MT）的比率荧光/比色法/智能手机三模式传感器。具体而言，具有良好发光特性和类过氧化物酶活性的Ru-CN催化邻苯二胺（OPD）氧化生成发光的二氨基联苯胺（DAP），由于荧光内滤效应（IFE），DAP反过来抑制Ru-CN的固有荧光。MT可抑制这一催化过程，导致Ru-CN荧光部分恢复，反应体系紫外可见信号降低，反应溶液黄色逐渐褪去。因此，该三模式传感器能够实现对MT的精确、可靠且高通量测定，相关检测限分别为0.32、0.46和0.67 μM。此外，该三模式传感器已应用于多种果汁样品中MT的检测，回收率在86.00%至99.68%之间，这表明该新方法适用于食品分析多模式光学传感器的研发。
