Sureau P, Perrin P, Horaud F
Dev Biol Stand. 1985;60:133-9.
From the trypsinized skin of a rabbit embryo, a fibroblastic cell line has been obtained (FEL cells). The primary seed lot has been established at 11th passage. The tests for tumorigenicity on nu/nu mice were negative (10(4) cell/animal) at passage level 11th and 50th. Cell growth and morphology were not altered when 100 passages were performed. FEL cells were tested for their susceptibility to rabies virus. The production of virus on FEL cells was investigated after infection by the P.V. rabies virus strain adapted to several cell types (bovine fetal kidney, Vero and BHK cells). For the evaluation of an experimental rabies vaccine produced with this substrate, FEL cells were compared to Vero cells. The synthesis of glycoprotein (EIA) and the immunogenicity (NIH test) were similar for the vaccines obtained with both cell systems. FEL cells could therefore be an alternative to Vero cells for the production of a cell culture inactivated rabies vaccine.
从兔胚胎胰蛋白酶消化后的皮肤中获得了一种成纤维细胞系（FEL细胞）。原代种子批在第11代时建立。在第11代和第50代时，对无胸腺裸鼠进行的致瘤性试验呈阴性（每只动物接种10⁴个细胞）。进行100代培养后，细胞生长和形态未发生改变。检测了FEL细胞对狂犬病病毒的敏感性。在用适应多种细胞类型（牛胎儿肾细胞、Vero细胞和BHK细胞）的P.V.狂犬病病毒株感染后，研究了FEL细胞上病毒的产生情况。为评估用该基质生产的实验性狂犬病疫苗，将FEL细胞与Vero细胞进行了比较。两种细胞系统获得的疫苗中糖蛋白的合成（酶免疫分析）和免疫原性（NIH试验）相似。因此，FEL细胞可作为Vero细胞的替代物用于生产细胞培养灭活狂犬病疫苗。
