用于检测甜菜坏死黄脉病毒的单克隆酶联免疫吸附测定法。

Monoclonal ELISA for the determination of BNYV-virus.

作者信息

Merten O W, Heberle-Bors E, Himmler G, Reiter S, Messner P, Katinger H

出版信息

Dev Biol Stand. 1985;60:451-6.

Abstract

Monoclonal antibodies have been developed against the BNYV-virus (beet-necrotic-yellow-vein) which causes the rhizomania disease in sugar beet (Beta vulgaris). The B103 hybridoma produced predominantly IgG2a at greater than 100 micrograms/ml. IgM-production could not, however, be ruled out. In a polyclonal-monoclonal indirect sandwich ELISA, the B103-antibody bound to BNYVV of Austrian, German, French and Bulgarian origin at a sensitivity of 1 ng/ml or less. Comparison of sugar beet samples in ELISA and immuno-electronmicroscopy showed good correlation, demonstrating the good performance of B103 antibody for broad application in BNYVV diagnosis.

摘要

已经开发出针对导致甜菜（Beta vulgaris）根瘤病的BNYV病毒（甜菜坏死黄脉病毒）的单克隆抗体。B103杂交瘤以大于100微克/毫升的浓度主要产生IgG2a。然而，不能排除IgM的产生。在多克隆-单克隆间接夹心ELISA中，B103抗体以1纳克/毫升或更低的灵敏度与奥地利、德国、法国和保加利亚来源的BNYVV结合。ELISA和免疫电子显微镜对甜菜样品的比较显示出良好的相关性，证明了B103抗体在BNYVV诊断中广泛应用的良好性能。