Li Zhongxu, Cheng Caihong, Zhang Li, Xue Jialin, Sun Qingyang, Wang Haoran, Cui Ruiguo, Liu Ruijie, Song Lijun
College of Food Science and Technology, Hebei Normal University of Science & Technology, Qinhuangdao 066612, China.
Hebei Key Laboratory of Natural Products Activity Components and Function, Hebei Normal University of Science & Technology, Qinhuangdao 066004, China.
Food Chem. 2025 Oct 1;488:144916. doi: 10.1016/j.foodchem.2025.144916. Epub 2025 May 24.
The chestnut processing industry produces a large amount of chestnut shells, which are usually discarded, resulting in a significant waste of biomass resources. In this study, chestnut shell pigments (CSP) were extracted using ultrasound-assisted deep eutectic solvent (DES) extraction. The choline chloride-lactic acid was selected as the optimal solvent. The total polyphenols content (TPC), DPPH, and FRAP were 43.83 mg GAE/g DW, 91.31 %, and 0.86 μmol/mL under the optimum conditions (61 °C, 21 %, 61 min). A total of 36 compounds were qualitatively determined in CSP using HPLC-MS. Calomelanol F, dihydrokaempferol, catechin, procyanidin B2, procyanidin A2, ellagic acid, isorhamnetin-3-O-glucoside, and coumarin derivatives were the dominant compounds. Compared with DES-3 (choline chloride -urea) and ethanol, DES-1 extract exhibited significantly higher color stability and antioxidant activity. FTIR and SEM analysis revealed that DES-1 formed more hydrogen bonds and showed stronger erosion effect, thereby enhancing the stability and extraction rate. This study provides a green and efficient alternative method for extracting natural pigments from chestnut shells.
板栗加工业产生大量的板栗壳，这些板栗壳通常被丢弃，导致生物质资源的大量浪费。在本研究中，采用超声辅助深共熔溶剂（DES）萃取法提取板栗壳色素（CSP）。选择氯化胆碱-乳酸作为最佳溶剂。在最佳条件（61℃、21%、61分钟）下，总多酚含量（TPC）、DPPH和FRAP分别为43.83毫克没食子酸当量/克干重、91.31%和0.86微摩尔/毫升。使用高效液相色谱-质谱联用（HPLC-MS）对CSP中的36种化合物进行了定性测定。甘汞醇F、二氢山奈酚、儿茶素、原花青素B2、原花青素A2、鞣花酸、异鼠李素-3-O-葡萄糖苷和香豆素衍生物是主要化合物。与DES-3（氯化胆碱-尿素）和乙醇相比，DES-1提取物表现出显著更高的颜色稳定性和抗氧化活性。傅里叶变换红外光谱（FTIR）和扫描电子显微镜（SEM）分析表明，DES-1形成了更多的氢键，显示出更强的侵蚀作用，从而提高了稳定性和提取率。本研究为从板栗壳中提取天然色素提供了一种绿色高效的替代方法。
