An Yuting, Yang Dongmei, Ma Bo, Liu Dongming, Deng Kaidong, Tao Shuanglun, Cai Huijuan, Ou Yangjing, Li Junnian
College of Biological Resources and Environmental Sciences, Jishou University, Jishou, 416000, China.
Department of Human Nutrition, Foods, and Exercise, Virginia Tech, Blacksburg, VA, 24061, USA.
J Chem Ecol. 2025 May 28;51(3):61. doi: 10.1007/s10886-025-01614-1.
Quercetin, a widely occurring phytogenic flavonoid, is the primary secondary metabolite in the diet of Microtus fortis. This study investigated the effects of dietary quercetin on immune organ development and cytokine levels in Microtus fortis. Microtus fortis were fed a standard diet containing 0, 1.5%, 3%, 5%, or 8% quercetin for 20 consecutive days. The results show that, compared to the control group, treatment with 5% quercetin increased the spleen and thymus indices by 14.47% and 11.67%, respectively. The proliferation of T lymphocytes, B lymphocytes and macrophages in Microtus fortis varied significantly cross groups, depending on the concentrations of quercetin in the culture medium. Additionally, IL-2 and IL-10 secretion levels increased by 8.90% and 14.99%, while TNF-α and IL-1β decreased by 11.13% and 17.40%. However, at an 8% quercetin concentration, compared with the 5% treatment, the indices of spleen and thymus decreased by 9.09% and 7.79%, respectively Similarly, IL-2 and IL-10 secretion levels decreased by 8.86% and 5.34%, while TNF-α and IL-1β secretion increased by 10.7% and 6.22%, respectively. These findings suggest that low concentrations of quercetin promote immune organ development and cytokine secretion in Microtus fortis, whereas higher concentrations exert inhibitory effects.
槲皮素是一种广泛存在的植物源黄酮类化合物，是东方田鼠饮食中的主要次生代谢产物。本研究调查了日粮中槲皮素对东方田鼠免疫器官发育和细胞因子水平的影响。将东方田鼠连续20天饲喂含0%、1.5%、3%、5%或8%槲皮素的标准日粮。结果表明，与对照组相比，5%槲皮素处理使脾脏和胸腺指数分别增加了14.47%和11.67%。东方田鼠中T淋巴细胞、B淋巴细胞和巨噬细胞的增殖在不同组间有显著差异，这取决于培养基中槲皮素的浓度。此外，IL-2和IL-10的分泌水平分别增加了8.90%和14.99%，而TNF-α和IL-1β分别下降了11.13%和17.40%。然而，在槲皮素浓度为8%时，与5%处理组相比，脾脏和胸腺指数分别下降了9.09%和7.79%。同样，IL-2和IL-10的分泌水平分别下降了8.86%和5.34%，而TNF-α和IL-1β的分泌分别增加了10.7%和6.22%。这些发现表明，低浓度的槲皮素促进东方田鼠免疫器官的发育和细胞因子的分泌，而高浓度则产生抑制作用。
