Ye Man-Hong, Nan Yan-Lei, Ding Meng-Meng, Hu Jun-Bang, Liu Qian, Wei Wan-Hong, Yang Sheng-Mei
College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China.
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China.
Comp Biochem Physiol B Biochem Mol Biol. 2016 Jun-Jul;196-197:19-26. doi: 10.1016/j.cbpb.2016.01.011. Epub 2016 Feb 2.
This study was designed to investigate the physiological and biochemical responses of Brandt's voles to the persistent presence of dietary tannic acid. The diet for animals in the experimental group was supplemented with 3% dietary tannic acid for 5weeks. The control group received a commercial lab chow. No significant differences were detected in body weight, organ (heart, kidney, and liver) weights, and organ parameters between animals from two groups. However, voles in the experimental group had significantly higher daily food intake, increased contents of proline and histidine in saliva and feces after protein hydrolysis, and elevated hepatic expression of transferrin than the control. Our results suggested the existence of adaptive strategies developed in Brandt's voles to overcome the adverse effects of dietary tannic acid. (1) Food consumption was increased to satisfy their nutritional demands. (2) The secretion of tannic-acid-binding salivary proteins was promoted. (3) The absorption of iron was enhanced. These alterations contributed to neutralize the negative effects of tannic acid and maintain body mass in animals supplemented with tannic acid. As the result of the consumption of tannic acid, hepatic expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase was significantly decreased, while the overall potential of the antioxidant system, characterized by increased hepatic enzymatic activities of catalase and glutathione peroxidase, was enhanced. Our results also implied the involvement of tannic acid in the regulation of lipid metabolism and oxidative stress in voles.
本研究旨在探究布氏田鼠对膳食中单宁酸持续存在的生理和生化反应。实验组动物的饮食中添加了3%的膳食单宁酸，持续5周。对照组则给予商业实验室饲料。两组动物在体重、器官（心脏、肾脏和肝脏）重量以及器官参数方面均未检测到显著差异。然而，实验组的田鼠每日食物摄入量显著更高，蛋白质水解后唾液和粪便中脯氨酸和组氨酸的含量增加，并且转铁蛋白的肝脏表达水平高于对照组。我们的结果表明，布氏田鼠形成了适应性策略来克服膳食单宁酸的不利影响。（1）增加食物消耗以满足其营养需求。（2）促进单宁酸结合唾液蛋白的分泌。（3）增强铁的吸收。这些改变有助于抵消单宁酸的负面影响，并维持补充单宁酸的动物的体重。由于摄入单宁酸，3-羟基-3-甲基戊二酰辅酶A还原酶的肝脏表达显著降低，而以过氧化氢酶和谷胱甘肽过氧化物酶的肝脏酶活性增加为特征的抗氧化系统的整体潜力增强。我们的结果还表明单宁酸参与了田鼠脂质代谢和氧化应激的调节。
