Dissanayake Dilani Chathurika, Chandrasekharan Naduviladath Vishvanath, Wijayarathna Champika Dilrukshi
Department of Chemistry, Faculty of Science, University of Colombo, Colombo 03, Sri Lanka.
University of Alabama at Birmingham, Birmingham, Alabama, USA.
Access Microbiol. 2025 May 27;7(5). doi: 10.1099/acmi.0.000954.v5. eCollection 2025.
This study explores the genomic basis of heavy metal resistance in strain TWSL_1, isolated from industrial textile effluent. The strain exhibited strong resistance to Cd², Pb² and Cu², with MICs of 50, 1,200 and 75 mg l, respectively. Whole-genome sequencing revealed a 2.66 Mb genome with 2,567 CDSs and a 99.81% average nucleotide identity to WS479. Comparative genomic analysis at the genus level revealed that strain TWSL_1 possesses a unique and expanded repertoire of heavy metal resistance genes, including the cadmium efflux system accessory protein and cadmium resistance protein, which are absent in pathogenic sp. used for the comparison. Phylogenetic analysis confirmed its classification within , with strong bootstrap support (100). Functional annotation highlighted metabolic versatility and stress response capabilities, supporting its adaptation to metal-rich environments. TWSL_1 demonstrated high Pb² removal efficiency, reducing concentrations by over 70%. These findings highlight TWSL_1 as a promising candidate for heavy metal bioremediation with potential applications in environmental detoxification and monitoring strategies.
本研究探索了从工业纺织废水中分离出的TWSL_1菌株重金属抗性的基因组基础。该菌株对Cd²、Pb²和Cu²表现出较强抗性，其最低抑菌浓度分别为50、1200和75 mg l。全基因组测序显示其基因组大小为2.66 Mb，有2567个编码序列，与WS479的平均核苷酸同一性为99.81%。属水平的比较基因组分析表明，TWSL_1菌株拥有独特且扩展的重金属抗性基因库，包括镉外排系统辅助蛋白和镉抗性蛋白，而用于比较的致病菌株中不存在这些基因。系统发育分析证实其在所属分类内，有很强的自展支持率（100）。功能注释突出了其代谢多样性和应激反应能力，支持其对富含金属环境的适应性。TWSL_1对Pb²的去除效率很高，能使浓度降低70%以上。这些发现突出了TWSL_1作为重金属生物修复的有前景候选者，在环境解毒和监测策略中具有潜在应用价值。
