Wang Zhe, Ma Qiaoli, Lin Fei
Department of Cardiology, Zibo First Hospital, No.121, Nanjing Road, Shandong, 255022, Zhangdian District, China.
Department of Cardiology, Zibo Central Hospital, Shandong, 255090, China.
J Cardiovasc Transl Res. 2025 May 29. doi: 10.1007/s12265-025-10625-y.
To investigate the role of miR-139-5p in acute coronary syndrome (ACS), serum miR-139-5p levels were measured via quantitative real-time polymerase chain reaction (qRT-PCR) in 117 ACS patients, 91 stable angina (SAP) patients, 89 healthy controls. Its associations with ACS severity, major adverse cardiovascular events (MACE) were evaluated using statistical tests, Kaplan-Meier survival, COX regression analysis. Effect of miR-139-5p on cell function and inflammation in oxidized low-density lipoprotein (ox-LDL) -induced human coronary artery smooth muscle cells (HCASMCs) was evaluated in vitro. Upregulated miR-134-5p in ACS distinguished ACS from SAP/health individuals, correlating with increased cardiac troponin I (cTnI), Gensini score, MACE risk. COX regression identified miR-139-5p as independent ACS prognostic factors. In vitro, miR-139-5p downregulation suppressed ox-LDL-induced HCASMC proliferation, migration, and inflammation. Upregulated miR-139-5p expression showed a diagnostic and prognostic value on ACS and was correlated with ACS severity. Downregulated miR-139-5p expression exhibited a suppressive effect on ACS progression.
为研究miR-139-5p在急性冠状动脉综合征（ACS）中的作用，采用定量实时聚合酶链反应（qRT-PCR）检测了117例ACS患者、91例稳定型心绞痛（SAP）患者及89例健康对照者的血清miR-139-5p水平。运用统计学检验、Kaplan-Meier生存分析及COX回归分析评估其与ACS严重程度、主要不良心血管事件（MACE）的相关性。体外评估miR-139-5p对氧化型低密度脂蛋白（ox-LDL）诱导的人冠状动脉平滑肌细胞（HCASMCs）细胞功能和炎症的影响。ACS中上调的miR-134-5p可将ACS与SAP/健康个体区分开来，与心肌肌钙蛋白I（cTnI）升高、Gensini评分及MACE风险相关。COX回归分析确定miR-139-5p为ACS独立的预后因素。在体外，下调miR-139-5p可抑制ox-LDL诱导的HCASMC增殖、迁移及炎症反应。上调的miR-139-5p表达对ACS具有诊断和预后价值，并与ACS严重程度相关。下调的miR-139-5p表达对ACS进展具有抑制作用。
