Javidmehr Davoud, Fesahat Farzaneh, Hassani Fatemeh, Talebi Ali Reza, Shahverdi Abdolhossein
Department of Reproductive Biology,Faculty of Basic Sciences and Advanced Technologies in Medicine,Royan Institute, ACECR, Tehran, Iran.
Reproductive Immunology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
JBRA Assist Reprod. 2025 Sep 22;29(3):411-417. doi: 10.5935/1518-0557.20250007.
The levels of cfDNA in the semen samples of infertile men and its relationship with the level of oxidative stress, antioxidant capacity and lipid peroxidation were investigated.
Total 100 semen samples were obtained from infertile men with abnormal sperm parameters (oligoasthenospermia (n=10), oligoteratozoospermia (n=10), oligoasthenoteratozoospermia (n=10), and non-obstructive azoospermia (n=50)) and normozoospermic men. cfDNA was extracted and quantified by qPCR. Different markers related to the oxidant and antioxidant status as well as oxidative stress were measured in the seminal plasma samples of the study groups.
cfDNAs content in the oligoasthenoteratozoospermia group was significantly higher than those in the controls. A significant decrease in cfDNAs was observed in the azoospermia group compared to the controls. There was a significant increase in malondialdehyde and other oxidant related markers in all patient groups rather than the normal individuals. In contrast, seminal plasma samples of all patients with abnormal semen parameters showed a significant reduction in the levels of factors associated with antioxidant capacity compared to the controls.
This study highlights the direct link between elevated cfDNA levels, oxidative stress, and impaired sperm parameters in men with azoospermia and oligospermia. Current data underscore the potential competence of cfDNA and oxidative stress as diagnostic tools to classify the severity of male infertility and abnormalities related to semen parameters. Our findings emphasize the importance of antioxidant therapy as an efficient strategy to reduce oxidative damage, enhance sperm quality, and improve reproductive outcomes for male partners of couples with infertility.
研究不育男性精液样本中游离DNA（cfDNA）水平及其与氧化应激水平、抗氧化能力和脂质过氧化的关系。
从精子参数异常的不育男性（少弱精子症（n = 10）、少畸精子症（n = 10）、少弱畸精子症（n = 10）和非梗阻性无精子症（n = 50））以及正常精子症男性中获取总共100份精液样本。通过qPCR提取并定量cfDNA。在研究组的精浆样本中测量与氧化剂和抗氧化状态以及氧化应激相关的不同标志物。
少弱畸精子症组中的cfDNA含量显著高于对照组。与对照组相比，无精子症组中观察到cfDNA显著减少。所有患者组而非正常个体中丙二醛和其他与氧化剂相关的标志物显著增加。相反，与对照组相比，所有精液参数异常患者的精浆样本中与抗氧化能力相关的因子水平显著降低。
本研究突出了无精子症和少精子症男性中cfDNA水平升高、氧化应激与精子参数受损之间的直接联系。当前数据强调了cfDNA和氧化应激作为诊断工具对男性不育严重程度和与精液参数相关异常进行分类的潜在能力。我们的研究结果强调了抗氧化治疗作为一种有效策略的重要性，该策略可减少氧化损伤、提高精子质量并改善不育夫妇男性伴侣的生殖结局。
