精索静脉曲张患者精液氧化还原电位、精子DNA片段与精液参数之间的关联
[Association among seminal oxidation-reduction potential, sperm DNA fragments and semen parameters in patients with varicocele].
作者信息
Guan Xiao-Chuan, Yu Yue-Xin, Zhang Ning, Zhou Jing, Yu Jia-Ping, Wang Yu, Liu Xing-Chi, Wang Bo-Lun
机构信息
Reproduction Medicine Center, General Hospital of Northern Theater Command, Shenyang, Liaoning 110003, China.
Department of Hyperbaric Oxygen Medicine , General Hospital of Northern Theater Command, Shenyang, Liaoning 110003, China.
出版信息
Zhonghua Nan Ke Xue. 2025 Jul;31(7):591-596.
OBJECTIVE
To investigate the relationship among seminal oxidation-reduction potential (nORP), sperm DNA fragmentation (DFI) and semen parameters in patients with varicocele.
METHODS
Clinical data of 522 patients treated in the reproductive andrology clinic of the Northern Theater General Hospital from November 2023 to December 2023 were retrospectively analyzed, including 435 men of childbearing age and 87 men of infertile age. The patients were divided into the varicocele group (n=116) and non-varicocele group (n=406) according to clinical diagnosis. The differences of seminal plasma nORP, DFI, sperm high DNA stain ability (HDS) and semen parameters were analyzed between the two groups. The relationship among general clinical data, seminal plasma nORP, semen parameters, DFI and HDS in patients with varicocele were further analyzed. According to the severity of varicocele, the patients were divided into three groups, including mild, moderate and severe. And the differences of seminal plasma nORP and semen parameters, DFI and HDS among all groups were analyzed. The differences of seminal plasma nORP, semen parameters, DFI and HDS were compared between the varicocele and non-varicocele groups.
RESULTS
The total sperm count, sperm concentration, progressive motility sperm percentage (PR%) and normal sperm morphology rate (NSMR) in patients with varicocele were significantly lower than those in control group (P<0.05). And seminal plasma nORP, DFI and HDS in patients with varicocele were significantly higher than those in control group (P<0.05). Seminal plasma nORP in patients with varicocele was significantly negatively correlated with total sperm, sperm concentration and NSMR (P<0.05), and significantly positively correlated with DFI and HDS (P<0.05). There were significant differences in nORP, total sperm count, sperm concentration, PR%, DFI and HDS among mild, moderate and severe varicocele groups (P<0.05). Seminal plasma nORP, sperm concentration, PR% and DFI in severe group were significantly lower than those in mild and moderate groups(P<0.05). Sperm count and HDS in severe group were significantly lower than those in mild group (P<0.05). In infertile patients, seminal plasma nORP, DFI and HDS in varicocele group were significantly higher than those in control group (P<0.05). And PR% in varicocele group was significantly lower than that in control group (P<0.05).
CONCLUSIONS
Seminal plasma nORP in patients with varicocele may be an important marker of oxidative stress affecting DFI and semen parameters.
目的
探讨精索静脉曲张患者精液氧化还原电位（nORP）、精子DNA碎片率（DFI）与精液参数之间的关系。
方法
回顾性分析2023年11月至2023年12月在北部战区总医院生殖男科门诊就诊的522例患者的临床资料，其中育龄男性435例，不育男性87例。根据临床诊断将患者分为精索静脉曲张组（n = 116）和非精索静脉曲张组（n = 406）。分析两组患者精液nORP、DFI、精子高DNA染色能力（HDS）及精液参数的差异。进一步分析精索静脉曲张患者一般临床资料、精液nORP、精液参数、DFI及HDS之间的关系。根据精索静脉曲张的严重程度将患者分为轻度、中度和重度三组，分析各组精液nORP、精液参数、DFI及HDS的差异。比较精索静脉曲张组与非精索静脉曲张组精液nORP、精液参数、DFI及HDS的差异。
结果
精索静脉曲张患者的精子总数、精子浓度、前向运动精子百分比（PR%）及正常精子形态率（NSMR）均显著低于对照组（P < 0.05）。精索静脉曲张患者的精液nORP、DFI及HDS均显著高于对照组（P < 0.05）。精索静脉曲张患者的精液nORP与精子总数、精子浓度及NSMR显著负相关（P < 0.05），与DFI及HDS显著正相关（P < 0.05）。轻度、中度和重度精索静脉曲张组之间的nORP、精子总数、精子浓度、PR%、DFI及HDS存在显著差异（P < 0.05）。重度组的精液nORP、精子浓度、PR%及DFI显著低于轻度和中度组（P < 0.05）。重度组的精子计数及HDS显著低于轻度组（P < 0.05）。在不育患者中,精索静脉曲张组的精液nORP、DFI及HDS显著高于对照组（P < 0.05）。精索静脉曲张组的PR%显著低于对照组（P < 0.05）。
结论
精索静脉曲张患者的精液nORP可能是影响DFI和精液参数的氧化应激的重要标志物。
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