Kanaeva Alika, Vodiasova Ekaterina, Ha Vo Thi, Uppe Viktoria, Dmitrieva Evgenija
A.O. Kovalevsky Institute of Biology of the Southern Seas, prosp. Leninsky 38, Moscow, Russia.
Coastal Branch of the Joint Vietnam-Russia Tropical Science and Technology Research Center, 30 Nguyen Thien Thuat st., Nha Trang City, Khanh Hoa province, Viet Nam.
Parasitol Int. 2025 Dec;109:103100. doi: 10.1016/j.parint.2025.103100. Epub 2025 May 27.
Monogeneans of the genus Calicotyle Diesing, 1850 were found in the cloaca of the Arafura skate Okamejei boesemani (Ishihara, 1987) from the coastal waters of Vietnam. A partial 28S rDNA sequence (822 bp) was obtained from the collected specimen and deposited in GenBank (accession number PP868284). Comparative analysis with the available 28S rDNA sequences of Calicotyle spp. in the NCBI database revealed the smallest p-distances with the C. kroyeri Diesing, 1850 species complex. However, the genetic distance between the obtained sequence and C. kroyeri (AF279746) from its type host, Amblyraja radiata (Donovan, 1808), and type locality (the North Sea), was 4.2 %, while distances from all C. kroyeri isolates from the Atlantic Ocean (AF279744 - AF279747) ranged from 4.2 to 5.9 %. Based on these results, a new species, C. confusa n. sp., was proposed. Morphologically, C. confusa n. sp. exhibits smaller, albeit overlapping, dimensions compared to C. kroyeri specimens from the North Atlantic. However, no significant differences were observed between the two species in taxonomically important characters. Further morphological studies of C. kroyeri from its type host, as well as from other hosts and regions, are required to identify potential diagnostic characters distinguishing it from C. confusa n. sp.
1850年命名的卡利科吸虫属（Calicotyle Diesing）的单殖吸虫，是在越南沿海水域的阿拉弗拉鳐（Okamejei boesemani (Ishihara, 1987)）泄殖腔中发现的。从采集的标本中获得了部分28S rDNA序列（822 bp），并将其存入GenBank（登录号PP868284）。与NCBI数据库中卡利科吸虫属（Calicotyle spp.）的现有28S rDNA序列进行比较分析，发现与1850年命名的克罗伊氏卡利科吸虫（C. kroyeri Diesing）物种复合体的p距离最小。然而，所获得的序列与其模式宿主辐射鳐（Amblyraja radiata (Donovan, 1808)）及模式产地（北海）的克罗伊氏卡利科吸虫（C. kroyeri (AF279746)）之间的遗传距离为4.2%，而与来自大西洋的所有克罗伊氏卡利科吸虫分离株（AF279744 - AF279747）的距离在4.2%至5.9%之间。基于这些结果，提出了一个新物种——混淆卡利科吸虫（C. confusa n. sp.）。在形态上，与来自北大西洋的克罗伊氏卡利科吸虫标本相比，混淆卡利科吸虫新物种的尺寸较小，尽管有重叠。然而，在分类学重要特征方面，未观察到这两个物种之间存在显著差异。需要对来自其模式宿主以及其他宿主和地区的克罗伊氏卡利科吸虫进行进一步的形态学研究，以确定将其与混淆卡利科吸虫新物种区分开来的潜在诊断特征。
