Ramírez-Cruz Edgar S, Pulido-Flores Griselda, Monks Scott, Martínez-Salazar Elizabeth A
Laboratorio de Morfología Animal, Centro de Investigaciones Biológicas, Ciudad del Conocimiento, Universidad Autónoma del Estado de Hidalgo, Colonia Carboneras, C. P. 42184, Mineral de la Reforma, Hidalgo, México.
Dirección de Laboratorios, Ciudad del Conocimiento, Universidad Autónoma del Estado de Hidalgo, Col. Carboneras, C. P. 42184, Mineral de la Reforma, Hidalgo, México.
J Parasitol. 2025 May 1;111(3):374-392. doi: 10.1645/24-19.
While surveying the parasites of the leopard frog Lithobates spectabilis in the Barranca de Metztitlán Biosphere Reserve, Hidalgo, Mexico, we found several specimens of an undescribed species of Halipegus (Trematoda: Derogenidae: Halipeginae) in the buccal cavity of this species of frog. The objective of this study was to describe the new species using an integrative approach, incorporating morphological data, multivariate analyses (principal and discriminant components), and phylogenetic analyses based on partial sequences of the conserved ribosomal regions (28S rDNA) of DNA and the mitochondrial subunit of the cytochrome c oxidase I gene (COI mtDNA). The methods of maximum likelihood, Bayesian inference, and maximum parsimony were used to determine the relationships of the new species to other hemiurids. Halipegus metztitlanensis n. sp. differs from its congeners mainly by the combination of the following characters: the distance from the ventral sucker to the right anterior testis, the distance from the ventral sucker to the left posterior testis, the length and width of the ovary, the width of the left testis, the distance between the oral sucker and the ventral sucker, and the length of the egg filament. The new species was recovered as a distinct group separate from other hemiuroideans based on the 28S rDNA and COI mtDNA phylogenetic analyses. This is the first report of a hemiurid trematode infecting the endemic leopard frog in Hidalgo, México and the second species described from the country.
在对墨西哥伊达尔戈州梅茨蒂特兰峡谷生物圈保护区的豹蛙（Lithobates spectabilis）寄生虫进行调查时，我们在这种蛙的口腔中发现了几种未描述过的哈利佩吸虫属（Halipegus）（吸虫纲：德氏科：哈利佩亚科）标本。本研究的目的是采用综合方法描述这个新物种，综合形态学数据、多变量分析（主成分分析和判别成分分析）以及基于DNA保守核糖体区域（28S rDNA）和细胞色素c氧化酶I基因线粒体亚基（COI mtDNA）部分序列的系统发育分析。使用最大似然法、贝叶斯推断法和最大简约法来确定新物种与其他半尾吸虫的关系。梅茨蒂特兰哈利佩吸虫（Halipegus metztitlanensis n. sp.）与其同属物种的主要区别在于以下特征的组合：腹吸盘到右前睾丸的距离、腹吸盘到左后睾丸的距离、卵巢的长度和宽度、左睾丸的宽度、口吸盘与腹吸盘之间的距离以及卵丝的长度。基于28S rDNA和COI mtDNA系统发育分析，新物种被确认为与其他半尾吸虫类不同的一个独特类群。这是关于一种半尾吸虫感染墨西哥伊达尔戈州特有豹蛙的首次报告，也是该国描述的第二个此类物种。
