Eshghi Samira, Jookar Kashi Fereshteh
Department of Cell and Molecular Biology, Faculty of Chemistry, University of Kashan, Ootb-E Ravandi Blvd, Kashan, Isfahan, Iran.
Sci Rep. 2025 May 29;15(1):18903. doi: 10.1038/s41598-025-03427-4.
In recent years, Cu nanoparticles (CuNPs) have emerged as a widely used and cost-effective tool, especially in analytical fields. This study investigates the biological properties of CuNPs synthesized by strain Cu1 and their efficiency in degrading Acid Red 88 dye. Additionally, it evaluates the potential of a novel combination of Marinospirillum alkaliphilum strain N and CuNPs for AR88 biodegradation. Strain Cu1, a copper-resistant strain from the Sungun copper mine, was used for biosynthesizing CuNPs, with 16S rDNA sequence analysis showing a 99.10% similarity to Micrococcus lylae. Characterization via UV-Vis, FTIR, XRD, and SEM-EDX confirmed that the CuNPs were spherical-shaped and ranged in size from 20 to 100 nm. These nanoparticles demonstrated antimicrobial effects against various bacteria. The cell free extract/CuNPs and biomass/CuNPs of the degrading Marinospirillum alkaliphilum strain N achieved 100% degradation of Acid Red 88 within 11 h. In contrast, cell free extract and the biomass of strain N alone required 24 h to achieve the same result. FTIR and GC-MS analyses verified the degradation of AR88 dye. Toxicity assessments using Artemia salina and radish seeds revealed that Acid Red 88 dye was toxic. In contrast, metabolite biodegradation and CuNPs exhibited low toxicity, no mortality of A. salina, and no inhibiting seed germination.
近年来，铜纳米颗粒（CuNPs）已成为一种广泛使用且具有成本效益的工具，尤其是在分析领域。本研究调查了菌株Cu1合成的CuNPs的生物学特性及其降解酸性红88染料的效率。此外，还评估了嗜碱海螺旋菌菌株N与CuNPs的新型组合对AR88生物降解的潜力。菌株Cu1是一种来自松贡铜矿的耐铜菌株，用于生物合成CuNPs，16S rDNA序列分析显示其与莱氏微球菌的相似度为99.10%。通过紫外可见光谱、傅里叶变换红外光谱、X射线衍射和扫描电子显微镜-能谱分析表征证实，CuNPs呈球形，尺寸范围为20至100纳米。这些纳米颗粒对多种细菌具有抗菌作用。降解性嗜碱海螺旋菌菌株N的无细胞提取物/CuNPs和生物质/CuNPs在11小时内实现了酸性红88的100%降解。相比之下，单独的菌株N的无细胞提取物和生物质需要24小时才能达到相同的结果。傅里叶变换红外光谱和气相色谱-质谱分析证实了AR88染料的降解。使用卤虫和萝卜种子进行的毒性评估表明，酸性红88染料具有毒性。相比之下，代谢物生物降解和CuNPs表现出低毒性，卤虫无死亡，种子发芽也未受抑制。
