School of Chemistry and Life Science, Suzhou University of Science and Technology, Suzhou, Jiangsu, 215009, China.
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, International Innovation Center for Forest Chemicals and Materials, College of Materials Science and Engineering, Nanjing Forestry University, Nanjing, Jiangsu, 210037, China.
Food Chem Toxicol. 2022 Oct;168:113367. doi: 10.1016/j.fct.2022.113367. Epub 2022 Aug 13.
Green fabrication of copper nanoparticles (CuNPs) is an environmentally friendly and cost-effective method of synthesis for biomedical and bioremediation applications. In recent times, bacterial pathogens contaminating or affecting food and food crops pose the greatest threat to the food industry. In addition to this issue, synthetic dyes released from the textile and dyeing industries are polluting aquatic ecosystems and agricultural lands. The combined impact of these two factors is considered a major threat to life. Therefore, the use of CuNPs will provide an effective and long-term solution as an antibacterial and dye removing agent. The current study focuses on the synthesis of CuNPs using the leaf extract of Chloroxylon swietenia (C-CuNPs). The formation of a peak at 390 nm and a change in color from yellow to dark brown confirmed the synthesis of C-CuNPs. Subsequent synthesis at pH 9 was suitable for preparing C-CuNPs. Structural and chemical characterization of C-CuNPs was performed using Fourier Transfer Infra-Red (FTIR), X-ray diffraction (XRD), Dynamic Light scattering (DLS), and Scanning Electron Microscopy (SEM) analysis. The synthesized C-CuNPs possess a crystalline nature, a functional group that resembles C. swietenia, and are negatively charged and spherical in shape. C-CuNPs were tested against Congo red, Coomassie blue, and crystal violet and they showed complete degradation within 24 h under optimum conditions. Disk diffusion and broth dilution assay were used to test the antibacterial activity of C-CuNPs against Staphylococcus nepalensis, Staphylococcus gallinarum, Pseudomonasstutzeri,Bacillus subtilis, and Enterococcus faecalis. Therefore, the present study represents the first report on C-CuNPs' ability to degrade synthetic dyes and kill foodborne bacterial pathogens. Thus, the study has shed light on the potential of green synthesized CuNPs as bioremediation and packaging material in the future.
铜纳米粒子（CuNPs）的绿色制造是一种用于生物医学和生物修复应用的环保且具有成本效益的合成方法。 近年来，污染或影响食品和粮食作物的细菌病原体对食品工业构成了最大威胁。 除了这个问题之外，纺织和染整工业释放的合成染料正在污染水生生态系统和农业用地。 这两个因素的共同影响被认为是对生命的主要威胁。 因此，使用 CuNPs 将作为一种抗菌和去除染料的试剂提供有效且长期的解决方案。 本研究侧重于使用 Chloroxylon swietenia 的叶提取物（C-CuNPs）合成 CuNPs。 在 390nm 处出现峰和颜色从黄色变为深褐色的变化证实了 C-CuNPs 的合成。 在 pH 9 下进行后续合成适合制备 C-CuNPs。 使用傅里叶变换红外（FTIR），X 射线衍射（XRD），动态光散射（DLS）和扫描电子显微镜（SEM）分析对 C-CuNPs 的结构和化学特性进行了表征。 合成的 C-CuNPs 具有结晶性质，类似于 C. swietenia 的官能团，并且带负电荷且呈球形。 C-CuNPs 被测试对刚果红，考马斯亮蓝和结晶紫的降解作用，在最佳条件下，它们在 24 小时内完全降解。 圆盘扩散和肉汤稀释测定用于测试 C-CuNPs 对尼泊尔葡萄球菌，鸡葡萄球菌，施氏假单胞菌，枯草芽孢杆菌和粪肠球菌的抗菌活性。 因此，本研究代表了关于 C-CuNPs 降解合成染料和杀死食源性病原体能力的首次报道。 因此，该研究揭示了绿色合成的 CuNPs 在未来作为生物修复和包装材料的潜力。
