Aboulthana Wael Mahmoud, Moussa Sherif Abdelmottaleb, Aziz Samir Wassef, Hussien Amal Gouda, Omara Enayat A, Bashandy Samir A E
Biochemistry Department, Biotechnology Research Institute, National Research Centre, 33 El Bohouth St, P.O. 12622, Dokki, Cairo, Egypt.
Pathology Department, National Research Centre, 33 El Bohouth St, P.O. 12622, Dokki, Cairo, Egypt.
Biol Trace Elem Res. 2025 May 30. doi: 10.1007/s12011-025-04673-3.
One of the atypical antipsychotic drugs is olanzapine (OLZ). Enhancing metabolic and detoxifying activities requires zinc (Zn). Thus, the objective of the current study was to determine if Zn supplementation might effectively reduce hepatic lesions induced by OLZ. The oxidative stress, inflammatory, and fibrotic indicators of the liver tissues were evaluated, and electrophoretic methods were used to analyze the native protein and isoenzyme patterns. Furthermore, the liver tissues were examined histopathologically. The dynamic motion of the extracted hemoglobin was also examined. In addition to the serious damage identified histopathologically, our results show that OLZ treatment altered the liver tissue's antioxidant and inflammatory indicators. The zinc sulfate (ZnSO) salt solution, given in a dose-dependent fashion, reduced these changes. Additionally, the ZnSO salt solution enhanced the natural protein, lipid, and calcium moieties of the protein pattern that were electrophoretically detected and changed by OLZ. In terms of the isoenzyme patterns, the ZnSO salt solution reduced the electrophoretic catalase (CAT), peroxidase (POX), and β-esterase (β-EST) isoenzyme patterns that were hampered by OLZ in a dose-dependent manner. Rats treated with OLZ in addition to ZnSO showed an increase in heme-heme interactions, suggesting that zinc therapy stabilized oxyhemoglobin. This promotes a more effective folding process that improves the use of oxygen. A dosage of 100 mg/kg of ZnSO was shown to normalize physiological, histological, and biochemical markers. It also improved interactions between heme molecules.
奥氮平（OLZ）是一种非典型抗精神病药物。增强代谢和解毒活性需要锌（Zn）。因此，本研究的目的是确定补充锌是否能有效减轻奥氮平诱导的肝脏损伤。评估了肝组织的氧化应激、炎症和纤维化指标，并采用电泳方法分析天然蛋白质和同工酶模式。此外，对肝组织进行了组织病理学检查。还检查了提取血红蛋白的动态运动。除了组织病理学上确定的严重损伤外，我们的结果表明，奥氮平治疗改变了肝组织的抗氧化和炎症指标。以剂量依赖方式给予的硫酸锌（ZnSO）盐溶液减少了这些变化。此外，ZnSO盐溶液增强了电泳检测到的、被奥氮平改变的蛋白质模式中的天然蛋白质、脂质和钙部分。就同工酶模式而言，ZnSO盐溶液以剂量依赖方式减少了被奥氮平阻碍的电泳过氧化氢酶（CAT）、过氧化物酶（POX）和β-酯酶（β-EST）同工酶模式。除ZnSO外还接受奥氮平治疗的大鼠显示血红素-血红素相互作用增加，这表明锌疗法稳定了氧合血红蛋白。这促进了更有效的折叠过程，从而改善了氧气的利用。结果表明，100mg/kg的ZnSO剂量可使生理、组织学和生化指标恢复正常。它还改善了血红素分子之间的相互作用。
